Photoactivation of a substrate for cell adhesion under standard fluorescence microscopes

Jun Nakanishi, Yukiko Kikuchi, Tohru Takarada, Hidekazu Nakayama, Kazuo Yamaguchi, Mizuo Maeda

Research output: Contribution to journalArticle

149 Citations (Scopus)

Abstract

Cell-culturing substrates where cell adhesion can be switched on by external stimuli during cell cultivation are useful scaffolds for tissue engineering, cell-based drug screening, and fundamental cellular studies. Here, we show a new strategy for photoactivation of a substrate for cell adhesion under standard fluorescence microscopes. A glass substrate chemically modified with an alkylsiloxane having a photocleavable 2-nitrobenzyl group was coated with bovine serum albumin to prevent cell adhesion. Upon irradiation under a fluorescence microscope, the protein was replaced with fibronectin, which made the irradiated region cell-adhesive. Subsequent seeding of HEK293 or COS7 cells produced patterns corresponding to the irradiated patterns. We succeeded for the first time in positioning single cells in proximity to cultivating single cells. The present method provides a general strategy for positioning single cells of same or different types at any locations on the substrate and will be useful for studying cell-cell interactions.

Original languageEnglish
Pages (from-to)16314-16315
Number of pages2
JournalJournal of the American Chemical Society
Volume126
Issue number50
DOIs
Publication statusPublished - 2004 Dec 22
Externally publishedYes

Fingerprint

Cell adhesion
Cell Adhesion
Microscopes
Fluorescence
Substrates
Scaffolds (biology)
Bovine Serum Albumin
Fibronectins
Tissue engineering
Adhesives
Screening
Irradiation
Proteins
Glass
Preclinical Drug Evaluations
Pharmaceutical Preparations
Tissue Engineering
Cell Communication

ASJC Scopus subject areas

  • Chemistry(all)

Cite this

Photoactivation of a substrate for cell adhesion under standard fluorescence microscopes. / Nakanishi, Jun; Kikuchi, Yukiko; Takarada, Tohru; Nakayama, Hidekazu; Yamaguchi, Kazuo; Maeda, Mizuo.

In: Journal of the American Chemical Society, Vol. 126, No. 50, 22.12.2004, p. 16314-16315.

Research output: Contribution to journalArticle

Nakanishi, Jun ; Kikuchi, Yukiko ; Takarada, Tohru ; Nakayama, Hidekazu ; Yamaguchi, Kazuo ; Maeda, Mizuo. / Photoactivation of a substrate for cell adhesion under standard fluorescence microscopes. In: Journal of the American Chemical Society. 2004 ; Vol. 126, No. 50. pp. 16314-16315.
@article{61506cc7649b4e86ba53638b79dcea77,
title = "Photoactivation of a substrate for cell adhesion under standard fluorescence microscopes",
abstract = "Cell-culturing substrates where cell adhesion can be switched on by external stimuli during cell cultivation are useful scaffolds for tissue engineering, cell-based drug screening, and fundamental cellular studies. Here, we show a new strategy for photoactivation of a substrate for cell adhesion under standard fluorescence microscopes. A glass substrate chemically modified with an alkylsiloxane having a photocleavable 2-nitrobenzyl group was coated with bovine serum albumin to prevent cell adhesion. Upon irradiation under a fluorescence microscope, the protein was replaced with fibronectin, which made the irradiated region cell-adhesive. Subsequent seeding of HEK293 or COS7 cells produced patterns corresponding to the irradiated patterns. We succeeded for the first time in positioning single cells in proximity to cultivating single cells. The present method provides a general strategy for positioning single cells of same or different types at any locations on the substrate and will be useful for studying cell-cell interactions.",
author = "Jun Nakanishi and Yukiko Kikuchi and Tohru Takarada and Hidekazu Nakayama and Kazuo Yamaguchi and Mizuo Maeda",
year = "2004",
month = "12",
day = "22",
doi = "10.1021/ja044684c",
language = "English",
volume = "126",
pages = "16314--16315",
journal = "Journal of the American Chemical Society",
issn = "0002-7863",
publisher = "American Chemical Society",
number = "50",

}

TY - JOUR

T1 - Photoactivation of a substrate for cell adhesion under standard fluorescence microscopes

AU - Nakanishi, Jun

AU - Kikuchi, Yukiko

AU - Takarada, Tohru

AU - Nakayama, Hidekazu

AU - Yamaguchi, Kazuo

AU - Maeda, Mizuo

PY - 2004/12/22

Y1 - 2004/12/22

N2 - Cell-culturing substrates where cell adhesion can be switched on by external stimuli during cell cultivation are useful scaffolds for tissue engineering, cell-based drug screening, and fundamental cellular studies. Here, we show a new strategy for photoactivation of a substrate for cell adhesion under standard fluorescence microscopes. A glass substrate chemically modified with an alkylsiloxane having a photocleavable 2-nitrobenzyl group was coated with bovine serum albumin to prevent cell adhesion. Upon irradiation under a fluorescence microscope, the protein was replaced with fibronectin, which made the irradiated region cell-adhesive. Subsequent seeding of HEK293 or COS7 cells produced patterns corresponding to the irradiated patterns. We succeeded for the first time in positioning single cells in proximity to cultivating single cells. The present method provides a general strategy for positioning single cells of same or different types at any locations on the substrate and will be useful for studying cell-cell interactions.

AB - Cell-culturing substrates where cell adhesion can be switched on by external stimuli during cell cultivation are useful scaffolds for tissue engineering, cell-based drug screening, and fundamental cellular studies. Here, we show a new strategy for photoactivation of a substrate for cell adhesion under standard fluorescence microscopes. A glass substrate chemically modified with an alkylsiloxane having a photocleavable 2-nitrobenzyl group was coated with bovine serum albumin to prevent cell adhesion. Upon irradiation under a fluorescence microscope, the protein was replaced with fibronectin, which made the irradiated region cell-adhesive. Subsequent seeding of HEK293 or COS7 cells produced patterns corresponding to the irradiated patterns. We succeeded for the first time in positioning single cells in proximity to cultivating single cells. The present method provides a general strategy for positioning single cells of same or different types at any locations on the substrate and will be useful for studying cell-cell interactions.

UR - http://www.scopus.com/inward/record.url?scp=11444252407&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=11444252407&partnerID=8YFLogxK

U2 - 10.1021/ja044684c

DO - 10.1021/ja044684c

M3 - Article

VL - 126

SP - 16314

EP - 16315

JO - Journal of the American Chemical Society

JF - Journal of the American Chemical Society

SN - 0002-7863

IS - 50

ER -