Photochemical system for regenerating NADPH from NADP with use of immobilized chloroplasts

Isao Karube*, Toshiaki Otsuka, Hiromichi Kayano, Tadashi Matsunaga, Shuichi Suzuki

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

16 Citations (Scopus)


Spinach chloroplasts were immobilized in 2% agar gel. Crude ferredoxin and NADP–ferredoxin oxidoreductase isolated from spinach were used as electron carriers. The activity of the NADP reduction by immobilized chloroplasts increased with increasing ferredoxin concentration and the maximum activity was obtained at 8μM ferredoxin. The saturation of NADP reduction was observed at a light intensity of over 1000 lx. The optimum pH and temperature of NADP reduction were 8 and 25°C, respectively. The reduced NADP in a reaction medium increased linearly with increasing reaction time under illumination. NADP was continuously reduced for 2 hr with a hollow‐fiber reactor containing immobilized chloroplasts. NADPH and NADP were separated with a hollow‐fiber dialyzer from ferredoxin and NADP–ferredoxin oxidoreductase, which were reused. The conversion ratio of NADP to NADPH was from 40 to 80%.

Original languageEnglish
Pages (from-to)2655-2665
Number of pages11
JournalBiotechnology and Bioengineering
Issue number12
Publication statusPublished - 1980
Externally publishedYes

ASJC Scopus subject areas

  • Biotechnology
  • Bioengineering
  • Applied Microbiology and Biotechnology


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