Photocontrol of cell adhesion on amino-bearing surfaces by reversible conjugation of poly(ethylene glycol) via a photocleavable linker

Shingo Kaneko, Hidekazu Nakayama, Yutaro Yoshino, Daisuke Fushimi, Kazuo Yamaguchi, Yasuhiro Horiike, Jun Nakanishi

Research output: Contribution to journalArticle

43 Citations (Scopus)

Abstract

Dynamic control of cell adhesion on substrates is a useful technology in tissue engineering and basic biology. This paper describes a method for the control of cell adhesion on amino-bearing surfaces by reversible conjugation of an anti-fouling polymer, poly(ethylene glycol) (PEG), via a newly developed photocleavable linker, 1-(5-methoxy-2-nitro-4-prop-2-ynyloxyphenyl)ethyl N-succinimidyl carbonate (1). This molecule has alkyne and succinimidyl carbonate at each end, which are connected by photocleavable 2-nitrobenzyl ester. Under this molecular design, the molecule crosslinked azides and amines, whose linkage cleaved upon application of near-UV light. By using aminosilanised glass and silicon as model substrates, we studied their reversible surface modification with PEG-azide (Mw = 5000) based on contact angle measurements, ellipsometry, and AFM morphological observations. Protein adsorption and cell adhesion dramatically changed by PEGylation and the following irradiation, which can be used for cellular patterning. Also, the capability of the substrate to change cell adhesiveness by photoirradiation during cell cultivation was demonstrated by inducing cell migration. We believe this method will be useful for dynamic patterning of cells on protein-based scaffolds.

Original languageEnglish
Pages (from-to)4051-4059
Number of pages9
JournalPhysical Chemistry Chemical Physics
Volume13
Issue number9
DOIs
Publication statusPublished - 2011 Mar 7
Externally publishedYes

Fingerprint

Bearings (structural)
Cell adhesion
conjugation
Polyethylene glycols
glycols
adhesion
ethylene
Azides
Substrates
cells
Molecules
Alkynes
Ellipsometry
Silicon
Angle measurement
Fouling
Tissue engineering
Scaffolds
Ultraviolet radiation
Contact angle

ASJC Scopus subject areas

  • Physics and Astronomy(all)
  • Physical and Theoretical Chemistry

Cite this

Photocontrol of cell adhesion on amino-bearing surfaces by reversible conjugation of poly(ethylene glycol) via a photocleavable linker. / Kaneko, Shingo; Nakayama, Hidekazu; Yoshino, Yutaro; Fushimi, Daisuke; Yamaguchi, Kazuo; Horiike, Yasuhiro; Nakanishi, Jun.

In: Physical Chemistry Chemical Physics, Vol. 13, No. 9, 07.03.2011, p. 4051-4059.

Research output: Contribution to journalArticle

Kaneko, Shingo ; Nakayama, Hidekazu ; Yoshino, Yutaro ; Fushimi, Daisuke ; Yamaguchi, Kazuo ; Horiike, Yasuhiro ; Nakanishi, Jun. / Photocontrol of cell adhesion on amino-bearing surfaces by reversible conjugation of poly(ethylene glycol) via a photocleavable linker. In: Physical Chemistry Chemical Physics. 2011 ; Vol. 13, No. 9. pp. 4051-4059.
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