Photodynamic diagnosis of oral carcinoma using talaporfin sodium and a hyperspectral imaging system: An animal study

Masashi Migita, Isao Kamiyama, Kenichi Matsuzaka, Atsushi Nakamura, Takayuki Souta, Katsuo Aizawa, Takahiko Shibahara

Research output: Contribution to journalArticle

3 Citations (Scopus)

Abstract

The purpose of this study was to examine the efficacy of detecting oral squamous cell carcinoma (SCC) using photodynamic diagnosis (PDD), a highly reliable method for tumor diagnosis, through the use of photosensitizers such as talaporfin sodium. Talaporfin sodium (5mg/kg) was administered to 7 control rats and 43 rats that had ingested the carcinogen 4-nitroquinoline 1-oxide (4NQO). After 8h, tongues were examined by spectral imaging and measurements were made using a hyperspectral imaging system. On each spectral image, macroscopically lesions and normal regions of the tongue were selected. Lesions comprised squamous cell carcinoma (SCC) in 22 cases, carcinoma in situ (CiS) in 9 cases, and dysplasia in 12 cases. The value Δα was determined as the absorbance spectrum of a lesion compared with normal tissue, or as the difference between Δκ at the posterior of the lingual protrusion and Δκ at the anterior of the lingual protrusion in controls. We compared Δα for SCC, CiS, and dysplasia with controls. The value Δα was (2.4±1.78)×10-2 in SCC, (1.2±1.34)×10-2 in CiS, (-0.2±0.49)×10-2 in dysplasia, and (0.21±0.63)×10-2 in controls. Both SCC and CiS showed significant differences from controls (p=0.037 and p=0.021, respectively). Histopathological analysis revealed that proliferating cell nuclear antigen (PCNA) expression increased gradually in the following order: controls, dysplasia, CiS, and SCC. Development of PDD using a hyperspectral imaging system may represent a useful technique for detecting oral SCC.

Original languageEnglish
Pages (from-to)126-132
Number of pages7
JournalAsian Journal of Oral and Maxillofacial Surgery
Volume22
Issue number3
DOIs
Publication statusPublished - 2010 Sep 1

Keywords

  • Hyperspectral imaging system
  • Oral squamous cell carcinoma
  • Photodynamic diagnosis
  • Talaporfin sodium

ASJC Scopus subject areas

  • Surgery
  • Oral Surgery
  • Orthopedics and Sports Medicine

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