Photolithographic coating of polymers on DNA as an approach for construction of nano-structures

D. Umeno, M. Kawasaki, M. Maeda

Research output: Contribution to journalConference articlepeer-review

3 Citations (Scopus)

Abstract

We synthesized poly(N-isopropylacrylamide) terminated with psoralen. Photo-induced reaction between DNA and the psoralen end group resulted in the grafting of poly(N-isopropylacrylamide) on double-stranded DNA. On the other hand, binding efficiency of DNA binding proteins such as restriction endonucleases to the DNA decreased significantly with increasing degree of polymer modification: At a certain level of polymer introduction, DNA was revealed to be practically 'jacketed'. Lithography was attempted to this grafting procedure. We found that pre-incubation of a restriction endonuclease EcoRI with DNA resulted in selective preservation of its binding site from polymer modification. The application of this technique would allow us facile methods to construct a precisely regulated nanoscale architecture.

Original languageEnglish
Pages (from-to)427-431
Number of pages5
JournalSupramolecular Science
Volume5
Issue number3-4
DOIs
Publication statusPublished - 1998
Externally publishedYes
EventProceedings of the 11th Toyota Conference on Nanostructured Materials in Biological and Artificial Systems - Shizuoka, Jpn
Duration: 1997 Nov 51997 Nov 8

ASJC Scopus subject areas

  • Engineering(all)

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