TY - JOUR
T1 - Photolithographic coating of polymers on DNA as an approach for construction of nano-structures
AU - Umeno, D.
AU - Kawasaki, M.
AU - Maeda, M.
N1 - Funding Information:
This work was supported in part by a Grant-in-Aid for ScientiÞc Research from the Ministry of Education, Science, Sports and Culture of Japan. Financial support by Kumagai Foundation for Science and Technology is also acknowledged.
PY - 1998
Y1 - 1998
N2 - We synthesized poly(N-isopropylacrylamide) terminated with psoralen. Photo-induced reaction between DNA and the psoralen end group resulted in the grafting of poly(N-isopropylacrylamide) on double-stranded DNA. On the other hand, binding efficiency of DNA binding proteins such as restriction endonucleases to the DNA decreased significantly with increasing degree of polymer modification: At a certain level of polymer introduction, DNA was revealed to be practically 'jacketed'. Lithography was attempted to this grafting procedure. We found that pre-incubation of a restriction endonuclease EcoRI with DNA resulted in selective preservation of its binding site from polymer modification. The application of this technique would allow us facile methods to construct a precisely regulated nanoscale architecture.
AB - We synthesized poly(N-isopropylacrylamide) terminated with psoralen. Photo-induced reaction between DNA and the psoralen end group resulted in the grafting of poly(N-isopropylacrylamide) on double-stranded DNA. On the other hand, binding efficiency of DNA binding proteins such as restriction endonucleases to the DNA decreased significantly with increasing degree of polymer modification: At a certain level of polymer introduction, DNA was revealed to be practically 'jacketed'. Lithography was attempted to this grafting procedure. We found that pre-incubation of a restriction endonuclease EcoRI with DNA resulted in selective preservation of its binding site from polymer modification. The application of this technique would allow us facile methods to construct a precisely regulated nanoscale architecture.
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U2 - 10.1016/S0968-5677(98)00044-3
DO - 10.1016/S0968-5677(98)00044-3
M3 - Conference article
AN - SCOPUS:0032108569
VL - 5
SP - 427
EP - 431
JO - Supramolecular Science
JF - Supramolecular Science
SN - 0968-5677
IS - 3-4
T2 - Proceedings of the 11th Toyota Conference on Nanostructured Materials in Biological and Artificial Systems
Y2 - 5 November 1997 through 8 November 1997
ER -