Photopatterning proteins and cells in aqueous environment using TiO2 photocatalysis

Hideaki Yamamoto, Takanori Demura, Kohei Sekine, Sho Kono, Michio Niwano, Ayumi Hirano-Iwata, Takashi Tanii

    Research output: Contribution to journalArticle

    2 Citations (Scopus)

    Abstract

    Organic contaminants adsorbed on the surface of titanium dioxide (TiO2) can be decomposed by photocatalysis under ultraviolet (UV) light. Here we describe a novel protocol employing the TiO2 photocatalysis to locally alter cell affinity of the substrate surface. For this experiment, a thin TiO2 film was sputter-coated on a glass coverslip, and the TiO2 surface was subsequently modified with an organosilane monolayer derived from octadecyltrichlorosilane (OTS), which inhibits cell adhesion. The sample was immersed in a cell culture medium, and focused UV light was irradiated to an octagonal region. When a neuronal cell line PC12 cells were plated on the sample, cells adhered only on the UV-irradiated area. We further show that this surface modification can also be performed in situ, i.e., even when cells are growing on the substrate. Proper modification of the surface required an extracellular matrix protein collagen to be present in the medium at the time of UV irradiation. The technique presented here can potentially be employed in patterning multiple cell types for constructing coculture systems or to arbitrarily manipulate cells under culture.

    Original languageEnglish
    Article numbere53045
    JournalJournal of Visualized Experiments
    Volume2015
    Issue number104
    DOIs
    Publication statusPublished - 2015 Oct 26

      Fingerprint

    Keywords

    • Bioengineering
    • Cell patterning
    • Issue 104
    • PC12 cells
    • Photocatalysis
    • Protein patterning
    • Self-assembled monolayer
    • Surface modification
    • Titanium dioxide

    ASJC Scopus subject areas

    • Biochemistry, Genetics and Molecular Biology(all)
    • Chemical Engineering(all)
    • Immunology and Microbiology(all)
    • Neuroscience(all)

    Cite this

    Yamamoto, H., Demura, T., Sekine, K., Kono, S., Niwano, M., Hirano-Iwata, A., & Tanii, T. (2015). Photopatterning proteins and cells in aqueous environment using TiO2 photocatalysis. Journal of Visualized Experiments, 2015(104), [e53045]. https://doi.org/10.3791/53045