Photosensitivities of iodopsin and rhodopsins.

Toshiyuki Okano, Y. Fukada, Y. Shichida, T. Yoshizawa

Research output: Contribution to journalArticle

48 Citations (Scopus)

Abstract

The relative photosensitivity and the molar extinction coefficient of a highly purified iodopsin (chicken red sensitive cone visual pigment) solubilized in a mixture of 3-[(3-cholamidopropyl)-dimethylammonio]-1-propanesulfonate and phosphatidylcholine (CHAPS-PC) were measured using bovine rhodopsin solubilized in 2% digitonin as a standard and compared with those of chicken and bovine rhodopsins. The photosensitivity obtained (1.08) was close to those of rhodopsins (chicken, 1.04; bovine, 0.99) in CHAPS-PC. The molar extinction coefficient of iodopsin (47,200) was 1.15-1.17 times higher than those of rhodopsins (chicken, 40,500; bovine, 41,200). The oscillator strength of iodopsin (0.60) calculated from the extinction coefficient was nearly identical to that of chicken rhodopsin (0.61), suggesting that the chromophore of iodopsin is similar in configuration to rhodopsin. In contrast, the difference in quantum yield between iodopsin (0.62) and chicken rhodopsin (0.70) suggests that the chromophore-opsin interaction after absorption of a photon by the chromophore may be different.

Original languageEnglish
Pages (from-to)995-1001
Number of pages7
JournalPhotochemistry and Photobiology
Volume56
Issue number6
Publication statusPublished - 1992 Dec
Externally publishedYes

Fingerprint

chickens
Photosensitivity
Rhodopsin
photosensitivity
Chickens
chromophores
Chromophores
extinction
Phosphatidylcholines
visual pigments
coefficients
Opsins
Digitonin
Retinal Pigments
oscillator strengths
Quantum yield
iodopsin
Photons
cones
Cones

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)
  • Biochemistry
  • Biophysics

Cite this

Okano, T., Fukada, Y., Shichida, Y., & Yoshizawa, T. (1992). Photosensitivities of iodopsin and rhodopsins. Photochemistry and Photobiology, 56(6), 995-1001.

Photosensitivities of iodopsin and rhodopsins. / Okano, Toshiyuki; Fukada, Y.; Shichida, Y.; Yoshizawa, T.

In: Photochemistry and Photobiology, Vol. 56, No. 6, 12.1992, p. 995-1001.

Research output: Contribution to journalArticle

Okano, T, Fukada, Y, Shichida, Y & Yoshizawa, T 1992, 'Photosensitivities of iodopsin and rhodopsins.', Photochemistry and Photobiology, vol. 56, no. 6, pp. 995-1001.
Okano T, Fukada Y, Shichida Y, Yoshizawa T. Photosensitivities of iodopsin and rhodopsins. Photochemistry and Photobiology. 1992 Dec;56(6):995-1001.
Okano, Toshiyuki ; Fukada, Y. ; Shichida, Y. ; Yoshizawa, T. / Photosensitivities of iodopsin and rhodopsins. In: Photochemistry and Photobiology. 1992 ; Vol. 56, No. 6. pp. 995-1001.
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