Phylogenetic position of turtles among reptiles: Evidence from immunological comparisons of eggshell matrices

Makiko Nishi, Isao Sarashina, Minoru Iijima, Ren Hirayama, Kazuyoshi Endo

Research output: Contribution to journalArticle

Abstract

The phylogenetic affiliation of turtles among major groups of reptiles is a controversial issue. Since turtles have no temporal fenestra unlike other reptiles and birds, turtles have been traditionally considered as the only survivors of anapsids, which have no fenestra, and as the most basal reptiles. Recent molecular studies, however, posit an archosaurian (crocodiles and birds) affinity of turtles and the earliest branching of squamates (lizards and snakes) instead of turtles. To address this issue, we performed enzyme linked immunosorbent assays (ELISA) on the extracts from eggshells of a total of 25 reptiles and birds. Antiserum raised against eggshell extracts from chicken (Galius gallus domesticus) reacted with those from crocodiles and turtles stronger than those from squamates, and the other antiserum raised against eggshell extracts from a soft-shelled turtle (Pelodiscus sinensis) reacted with those from birds and crocodiles stronger than those from squamates. These results support the archosaurian affinity of turtles and the scheme of recent molecular phylogeny.

Original languageEnglish
Pages (from-to)45-51
Number of pages7
JournalEarth Evolution Sciences
Volume3
Publication statusPublished - 2009 Mar 31

Fingerprint

eggshell
reptile
turtle
phylogenetics
matrix
bird
comparison
snake
lizard
phylogeny
assay
enzyme

Keywords

  • Anapsids
  • Eggshells
  • ELISA
  • Pelodiscus sinensis
  • Turtles

ASJC Scopus subject areas

  • Earth and Planetary Sciences(all)

Cite this

Phylogenetic position of turtles among reptiles : Evidence from immunological comparisons of eggshell matrices. / Nishi, Makiko; Sarashina, Isao; Iijima, Minoru; Hirayama, Ren; Endo, Kazuyoshi.

In: Earth Evolution Sciences, Vol. 3, 31.03.2009, p. 45-51.

Research output: Contribution to journalArticle

Nishi, Makiko ; Sarashina, Isao ; Iijima, Minoru ; Hirayama, Ren ; Endo, Kazuyoshi. / Phylogenetic position of turtles among reptiles : Evidence from immunological comparisons of eggshell matrices. In: Earth Evolution Sciences. 2009 ; Vol. 3. pp. 45-51.
@article{4e79aae508c341259452cc8a106091a8,
title = "Phylogenetic position of turtles among reptiles: Evidence from immunological comparisons of eggshell matrices",
abstract = "The phylogenetic affiliation of turtles among major groups of reptiles is a controversial issue. Since turtles have no temporal fenestra unlike other reptiles and birds, turtles have been traditionally considered as the only survivors of anapsids, which have no fenestra, and as the most basal reptiles. Recent molecular studies, however, posit an archosaurian (crocodiles and birds) affinity of turtles and the earliest branching of squamates (lizards and snakes) instead of turtles. To address this issue, we performed enzyme linked immunosorbent assays (ELISA) on the extracts from eggshells of a total of 25 reptiles and birds. Antiserum raised against eggshell extracts from chicken (Galius gallus domesticus) reacted with those from crocodiles and turtles stronger than those from squamates, and the other antiserum raised against eggshell extracts from a soft-shelled turtle (Pelodiscus sinensis) reacted with those from birds and crocodiles stronger than those from squamates. These results support the archosaurian affinity of turtles and the scheme of recent molecular phylogeny.",
keywords = "Anapsids, Eggshells, ELISA, Pelodiscus sinensis, Turtles",
author = "Makiko Nishi and Isao Sarashina and Minoru Iijima and Ren Hirayama and Kazuyoshi Endo",
year = "2009",
month = "3",
day = "31",
language = "English",
volume = "3",
pages = "45--51",
journal = "Earth Evolution Sciences",
issn = "1881-8463",
publisher = "University of Tsukuba",

}

TY - JOUR

T1 - Phylogenetic position of turtles among reptiles

T2 - Evidence from immunological comparisons of eggshell matrices

AU - Nishi, Makiko

AU - Sarashina, Isao

AU - Iijima, Minoru

AU - Hirayama, Ren

AU - Endo, Kazuyoshi

PY - 2009/3/31

Y1 - 2009/3/31

N2 - The phylogenetic affiliation of turtles among major groups of reptiles is a controversial issue. Since turtles have no temporal fenestra unlike other reptiles and birds, turtles have been traditionally considered as the only survivors of anapsids, which have no fenestra, and as the most basal reptiles. Recent molecular studies, however, posit an archosaurian (crocodiles and birds) affinity of turtles and the earliest branching of squamates (lizards and snakes) instead of turtles. To address this issue, we performed enzyme linked immunosorbent assays (ELISA) on the extracts from eggshells of a total of 25 reptiles and birds. Antiserum raised against eggshell extracts from chicken (Galius gallus domesticus) reacted with those from crocodiles and turtles stronger than those from squamates, and the other antiserum raised against eggshell extracts from a soft-shelled turtle (Pelodiscus sinensis) reacted with those from birds and crocodiles stronger than those from squamates. These results support the archosaurian affinity of turtles and the scheme of recent molecular phylogeny.

AB - The phylogenetic affiliation of turtles among major groups of reptiles is a controversial issue. Since turtles have no temporal fenestra unlike other reptiles and birds, turtles have been traditionally considered as the only survivors of anapsids, which have no fenestra, and as the most basal reptiles. Recent molecular studies, however, posit an archosaurian (crocodiles and birds) affinity of turtles and the earliest branching of squamates (lizards and snakes) instead of turtles. To address this issue, we performed enzyme linked immunosorbent assays (ELISA) on the extracts from eggshells of a total of 25 reptiles and birds. Antiserum raised against eggshell extracts from chicken (Galius gallus domesticus) reacted with those from crocodiles and turtles stronger than those from squamates, and the other antiserum raised against eggshell extracts from a soft-shelled turtle (Pelodiscus sinensis) reacted with those from birds and crocodiles stronger than those from squamates. These results support the archosaurian affinity of turtles and the scheme of recent molecular phylogeny.

KW - Anapsids

KW - Eggshells

KW - ELISA

KW - Pelodiscus sinensis

KW - Turtles

UR - http://www.scopus.com/inward/record.url?scp=84865676942&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=84865676942&partnerID=8YFLogxK

M3 - Article

AN - SCOPUS:84865676942

VL - 3

SP - 45

EP - 51

JO - Earth Evolution Sciences

JF - Earth Evolution Sciences

SN - 1881-8463

ER -