Physical exercise induces oxidation of plasma protein thiols to cysteine mixed disulfides in humans

Takayo Inayama, Misato Kashiba, Jun Oka, Mitsuru Higuchi, Keizo Umegaki, Makoto Saito, Yorihiro Yamamoto, Mitsuo Matsuda

Research output: Contribution to journalArticlepeer-review

2 Citations (Scopus)


We have reported that strenuous physical exercise causes a decrease in protein-bound sulfhydryl groups (p-SHs), such as albumin cysteine residues, in human plasma (Inayama et al., 1996, Life Sci., 59, 573-578). We further investigated the fate of plasma protein thiols after moderate exercise. Six untrained healthy female volunteers ran for 30-min at the individual ventilatory threshold. We observed an increase in protein cysteine mixed disulfides (p-S-Cys) after running, as evidenced by reducing plasma proteins with dithiothreitol to detect the increase of cysteine, along with the concomitant decrease in p-SHs in plasma. However, plasma protein-bound glutathione (GSH) and S-nitroso-protein were undetectable before and after exercise. Test tube experiments suggest that p-S-Cys are probably formed by the hydrolysis of protein GSH mixed disulfides by γ-glutamyltranspeptidase and peptidase, and/or by the oxidative addition of p-SHs to cystine.

Original languageEnglish
Pages (from-to)399-403
Number of pages5
JournalJournal of Health Science
Issue number5
Publication statusPublished - 2002 Oct
Externally publishedYes


  • Cysteine mixed disulfide
  • Human
  • Physical exercise
  • Plasma protein thiol

ASJC Scopus subject areas

  • Toxicology
  • Health, Toxicology and Mutagenesis


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