Physicochemical characters of a tyrosinase inhibitor produced by Streptomyces roseolilacinus NBRC 12815

Takuji Nakashima*, Kozue Anzai, Natsumi Kuwahara, Hisayuki Komaki, Shinji Miyadoh, Shigeaki Harayama, Ma Diarey Bordon Tianero, Junichi Tanaka, Akihiko Kanamoto, Katsuhiko Ando

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

13 Citations (Scopus)


We examined the biological activities present in Streptomyces strains preserved at the National Institute of Technology and Evaluation Biological Resource Center, and found a metabolite of Streptomyces roseolilacinus NBRC 12815 that showed a potent anti-tyrosinase activity. The compounds with anti-tyrosinase activity were pu- rifled by several chromatographic procedures. Final HPLC analysis revealed at least two anti-tyrosinase compounds with different retention times (12815A and B). The identification of two anti-tyrosinase compounds was performed with instrumental analysis and database search. The results obtained suggest that the active compounds are SF 2583A and B. Compound 12815A (IC50 values; about 9 hm) showed more potent tyrosinase inhibition than compound 12815B (IC50 values; about 1086 jUm). The only structural difference between 12815A and B is the presence of an additional chloric atom. In addition, the activity of 12815A was markedly decreased under acidic conditions, resulting in irreversible inactivation. However, the inactivated 12815A still exhibited residual activity when exposed to detergent, Tween 80. These results suggest that the chlorine and the hydration water are very important in the exertion of anti-tyrosinase activity by 12815A.

Original languageEnglish
Pages (from-to)832-836
Number of pages5
JournalBiological and Pharmaceutical Bulletin
Issue number5
Publication statusPublished - 2009 May
Externally publishedYes


  • Secondary metabolite
  • Straptomyces roseolilacinus
  • Tyrosinase inhibitor

ASJC Scopus subject areas

  • Pharmacology
  • Pharmaceutical Science


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