Physiologic capacity of reticuloendothelial system for degradation of Hb-vesicles (artificial oxygen carriers) after massive intravenous doses by daily repeated infusions for 14 days

Hiromi Sakai, Keitaro Sou, Shinji Takeoka, Hirohisa Horinouchi, Koichi Kobayashi, Eishun Tsuchida

Research output: Contribution to conferencePaper

Abstract

Hb-vesicles (HbV) are artificial O2 carriers as a transfusion alternative. We examined the physiological capacity for the degradation of HbV by daily repeated infusions (DRI). Male Wistar rats intravenously received the HbV at 10 mL/kg/day for 14 consecutive days. One day after DRI, hepatosplenomegaly occurred significantly through the accumulation of large amounts of HbV. Plasma clinical chemistry was overall normal, except for a transient elevation of lipid components derived from HbV. These symptoms subsided 14 days after DRI. The plasma iron and bilirubin levels remained unchanged, suggesting that the heme-degrading capacity of the RES did not surpass the ability to eliminate bilirubin. Accordingly, Hb encapsulation would be beneficial for heme detoxification through their preferential delivery to the RES, a physiologic compartment for degradation of senescent RBCs, even at doses greater than putative clinical doses.

Original languageEnglish
Number of pages1
Publication statusPublished - 2005 Dec 1
Event54th SPSJ Annual Meeting 2005 - Yokohama, Japan
Duration: 2005 May 252005 May 27

Conference

Conference54th SPSJ Annual Meeting 2005
CountryJapan
CityYokohama
Period05/5/2505/5/27

Keywords

  • Artificial red cells
  • Daily repeated infusion
  • Heme-detoxification
  • Liposome
  • MPS
  • RES

ASJC Scopus subject areas

  • Engineering(all)

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    Sakai, H., Sou, K., Takeoka, S., Horinouchi, H., Kobayashi, K., & Tsuchida, E. (2005). Physiologic capacity of reticuloendothelial system for degradation of Hb-vesicles (artificial oxygen carriers) after massive intravenous doses by daily repeated infusions for 14 days. Paper presented at 54th SPSJ Annual Meeting 2005, Yokohama, Japan.