Physiologic capacity of reticuloendothelial system for degradation of Hb-vesicles (artificial oxygen carriers) after massive intravenous doses by daily repeated infusions for 14 days

Hiromi Sakai, Keitaro Sou, Shinji Takeoka, Hirohisa Horinouchi, Koichi Kobayashi, Eishun Tsuchida

    Research output: Chapter in Book/Report/Conference proceedingConference contribution

    Abstract

    Hb-vesicles (HbV) are artificial O2 carriers as a transfusion alternative. We examined the physiological capacity for the degradation of HbV by daily repeated infusions (DRI). Male Wistar rats intravenously received the HbV at 10 mL/kg/day for 14 consecutive days. One day after DRI, hepatosplenomegaly occurred significantly through the accumulation of large amounts of HbV. Plasma clinical chemistry was overall normal, except for a transient elevation of lipid components derived from HbV. These symptoms subsided 14 days after DRI. The plasma iron and bilirubin levels remained unchanged, suggesting that the heme-degrading capacity of the RES did not surpass the ability to eliminate bilirubin. Accordingly, Hb encapsulation would be beneficial for heme detoxification through their preferential delivery to the RES, a physiologic compartment for degradation of senescent RBCs, even at doses greater than putative clinical doses.

    Original languageEnglish
    Title of host publicationPolymer Preprints, Japan
    Pages2272
    Number of pages1
    Volume54
    Edition1
    Publication statusPublished - 2005
    Event54th SPSJ Annual Meeting 2005 - Yokohama
    Duration: 2005 May 252005 May 27

    Other

    Other54th SPSJ Annual Meeting 2005
    CityYokohama
    Period05/5/2505/5/27

    Fingerprint

    Plasmas
    Degradation
    Detoxification
    Oxygen
    Encapsulation
    Lipids
    Rats
    Iron
    Clinical Chemistry

    Keywords

    • Artificial red cells
    • Daily repeated infusion
    • Heme-detoxification
    • Liposome
    • MPS
    • RES

    ASJC Scopus subject areas

    • Engineering(all)

    Cite this

    Sakai, H., Sou, K., Takeoka, S., Horinouchi, H., Kobayashi, K., & Tsuchida, E. (2005). Physiologic capacity of reticuloendothelial system for degradation of Hb-vesicles (artificial oxygen carriers) after massive intravenous doses by daily repeated infusions for 14 days. In Polymer Preprints, Japan (1 ed., Vol. 54, pp. 2272)

    Physiologic capacity of reticuloendothelial system for degradation of Hb-vesicles (artificial oxygen carriers) after massive intravenous doses by daily repeated infusions for 14 days. / Sakai, Hiromi; Sou, Keitaro; Takeoka, Shinji; Horinouchi, Hirohisa; Kobayashi, Koichi; Tsuchida, Eishun.

    Polymer Preprints, Japan. Vol. 54 1. ed. 2005. p. 2272.

    Research output: Chapter in Book/Report/Conference proceedingConference contribution

    Sakai, H, Sou, K, Takeoka, S, Horinouchi, H, Kobayashi, K & Tsuchida, E 2005, Physiologic capacity of reticuloendothelial system for degradation of Hb-vesicles (artificial oxygen carriers) after massive intravenous doses by daily repeated infusions for 14 days. in Polymer Preprints, Japan. 1 edn, vol. 54, pp. 2272, 54th SPSJ Annual Meeting 2005, Yokohama, 05/5/25.
    Sakai, Hiromi ; Sou, Keitaro ; Takeoka, Shinji ; Horinouchi, Hirohisa ; Kobayashi, Koichi ; Tsuchida, Eishun. / Physiologic capacity of reticuloendothelial system for degradation of Hb-vesicles (artificial oxygen carriers) after massive intravenous doses by daily repeated infusions for 14 days. Polymer Preprints, Japan. Vol. 54 1. ed. 2005. pp. 2272
    @inproceedings{63264296d79c465195b66dfd97c8c539,
    title = "Physiologic capacity of reticuloendothelial system for degradation of Hb-vesicles (artificial oxygen carriers) after massive intravenous doses by daily repeated infusions for 14 days",
    abstract = "Hb-vesicles (HbV) are artificial O2 carriers as a transfusion alternative. We examined the physiological capacity for the degradation of HbV by daily repeated infusions (DRI). Male Wistar rats intravenously received the HbV at 10 mL/kg/day for 14 consecutive days. One day after DRI, hepatosplenomegaly occurred significantly through the accumulation of large amounts of HbV. Plasma clinical chemistry was overall normal, except for a transient elevation of lipid components derived from HbV. These symptoms subsided 14 days after DRI. The plasma iron and bilirubin levels remained unchanged, suggesting that the heme-degrading capacity of the RES did not surpass the ability to eliminate bilirubin. Accordingly, Hb encapsulation would be beneficial for heme detoxification through their preferential delivery to the RES, a physiologic compartment for degradation of senescent RBCs, even at doses greater than putative clinical doses.",
    keywords = "Artificial red cells, Daily repeated infusion, Heme-detoxification, Liposome, MPS, RES",
    author = "Hiromi Sakai and Keitaro Sou and Shinji Takeoka and Hirohisa Horinouchi and Koichi Kobayashi and Eishun Tsuchida",
    year = "2005",
    language = "English",
    volume = "54",
    pages = "2272",
    booktitle = "Polymer Preprints, Japan",
    edition = "1",

    }

    TY - GEN

    T1 - Physiologic capacity of reticuloendothelial system for degradation of Hb-vesicles (artificial oxygen carriers) after massive intravenous doses by daily repeated infusions for 14 days

    AU - Sakai, Hiromi

    AU - Sou, Keitaro

    AU - Takeoka, Shinji

    AU - Horinouchi, Hirohisa

    AU - Kobayashi, Koichi

    AU - Tsuchida, Eishun

    PY - 2005

    Y1 - 2005

    N2 - Hb-vesicles (HbV) are artificial O2 carriers as a transfusion alternative. We examined the physiological capacity for the degradation of HbV by daily repeated infusions (DRI). Male Wistar rats intravenously received the HbV at 10 mL/kg/day for 14 consecutive days. One day after DRI, hepatosplenomegaly occurred significantly through the accumulation of large amounts of HbV. Plasma clinical chemistry was overall normal, except for a transient elevation of lipid components derived from HbV. These symptoms subsided 14 days after DRI. The plasma iron and bilirubin levels remained unchanged, suggesting that the heme-degrading capacity of the RES did not surpass the ability to eliminate bilirubin. Accordingly, Hb encapsulation would be beneficial for heme detoxification through their preferential delivery to the RES, a physiologic compartment for degradation of senescent RBCs, even at doses greater than putative clinical doses.

    AB - Hb-vesicles (HbV) are artificial O2 carriers as a transfusion alternative. We examined the physiological capacity for the degradation of HbV by daily repeated infusions (DRI). Male Wistar rats intravenously received the HbV at 10 mL/kg/day for 14 consecutive days. One day after DRI, hepatosplenomegaly occurred significantly through the accumulation of large amounts of HbV. Plasma clinical chemistry was overall normal, except for a transient elevation of lipid components derived from HbV. These symptoms subsided 14 days after DRI. The plasma iron and bilirubin levels remained unchanged, suggesting that the heme-degrading capacity of the RES did not surpass the ability to eliminate bilirubin. Accordingly, Hb encapsulation would be beneficial for heme detoxification through their preferential delivery to the RES, a physiologic compartment for degradation of senescent RBCs, even at doses greater than putative clinical doses.

    KW - Artificial red cells

    KW - Daily repeated infusion

    KW - Heme-detoxification

    KW - Liposome

    KW - MPS

    KW - RES

    UR - http://www.scopus.com/inward/record.url?scp=33645638711&partnerID=8YFLogxK

    UR - http://www.scopus.com/inward/citedby.url?scp=33645638711&partnerID=8YFLogxK

    M3 - Conference contribution

    VL - 54

    SP - 2272

    BT - Polymer Preprints, Japan

    ER -