Physiological sample uniformity and time-course stability in lined-up structure of human cardiomyocyte network for in vitro predictive drug-induced cardiotoxicity

Tomoyo Hamada, Tomoyuki Kaneko, Fumimasa Nomura, Kenji Yasuda

Research output: Contribution to journalArticle

Abstract

We have evaluated the electrophysiological characteristics of a line-shaped network of a three-dimensionally controlled in vitro human cardiomyocyte assay (hCM line) against conventional cell clusters as the standard model (hCM cluster) from the viewpoint of quality control of sample variety and time-course stability. The beating intervals of the hCM line demonstrated a more stable uniformity of samples (846 ×130 ms, 15.3% fluctuation) and better time-course stability, whereas those of the hCM cluster showed a much larger variety of samples (2001×1127 ms, 56.3% fluctuation) and weaker time-course stability. The field potential amplitude of the hCM line also showed better uniformity of samples (629×428-V, 68.0% fluctuation) against those of the hCM cluster (1984-2288-V, 115.3% fluctuation). The results suggested the importance of the cell-network shape control for the uniformity and stability of the beating interval and the field potential amplitude. They also suggest that the hCM line can improve the reproducibility and accuracy of the samples, which is important for a functional human cardiotoxicity model.

Original languageEnglish
Article number06GK05
JournalJapanese Journal of Applied Physics
Volume52
Issue number6 PART 2
DOIs
Publication statusPublished - 2013 Jun
Externally publishedYes

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ASJC Scopus subject areas

  • Engineering(all)
  • Physics and Astronomy(all)

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