Plasma membrane-localized Al-transporter from blue hydrangea sepals is a member of the anion permease family

Takashi Negishi, Kenshiro Oshima, Masahira Hattori, Kumi Yoshida

Research output: Contribution to journalArticle

15 Citations (Scopus)

Abstract

In hydrangea sepals, an aluminum complex of delphinidin-3-O-glucoside is responsible for the development of the blue color, and co-existing copigments mediate the solubilization and stabilization of the blue Al-anthocyanin complex which is localized in the sepal vacuole. In addition, hydrangeas are Al-hyperaccumulators and exhibit tolerance to acidic soils, in which the toxicity is due to soluble Al ion. Therefore, an Al-absorbing transport and storage system must exist in hydrangea. Recently, we cloned vacuolar and plasma membrane-localized Al-transporters, HmVALT, and HmPALT1, which are both members of the aquaporin family. However, HmPALT1 was only expressed in the sepals, indicating that a different Al-transporter should exist for absorption and long-distance transportation in the hydrangea plant. Using genetic information and microarray analysis, we identified an additional aluminum transporter gene, HmPALT2, which belongs to a member of the anion permease. The transcript was expressed in all tissues of hydrangea plants, and a transient expression study indicated that the gene product is localized to the plasma membrane. The results of an aluminum tolerance assay using yeast cells showed that the HmPALT2 is also involved in the transport of other metal(loid)s. The over-expression of HmPALT2 in Arabidopsis resulted in aluminum-hypersensitivity, suggesting that HmPALT2 should work as an aluminum transporter into cells in planta.

Original languageEnglish
Pages (from-to)341-352
Number of pages12
JournalGenes to Cells
Volume18
Issue number5
DOIs
Publication statusPublished - 2013 May
Externally publishedYes

Fingerprint

Hydrangea
Membrane Transport Proteins
Aluminum
Anions
Cell Membrane
Aquaporins
Anthocyanins
Microarray Analysis
Vacuoles
Arabidopsis
Genes
Hypersensitivity
Soil
Color
Yeasts
Metals
Ions

ASJC Scopus subject areas

  • Genetics
  • Cell Biology

Cite this

Plasma membrane-localized Al-transporter from blue hydrangea sepals is a member of the anion permease family. / Negishi, Takashi; Oshima, Kenshiro; Hattori, Masahira; Yoshida, Kumi.

In: Genes to Cells, Vol. 18, No. 5, 05.2013, p. 341-352.

Research output: Contribution to journalArticle

Negishi, Takashi ; Oshima, Kenshiro ; Hattori, Masahira ; Yoshida, Kumi. / Plasma membrane-localized Al-transporter from blue hydrangea sepals is a member of the anion permease family. In: Genes to Cells. 2013 ; Vol. 18, No. 5. pp. 341-352.
@article{3e8851dd36e643db8dbf35a725296aa1,
title = "Plasma membrane-localized Al-transporter from blue hydrangea sepals is a member of the anion permease family",
abstract = "In hydrangea sepals, an aluminum complex of delphinidin-3-O-glucoside is responsible for the development of the blue color, and co-existing copigments mediate the solubilization and stabilization of the blue Al-anthocyanin complex which is localized in the sepal vacuole. In addition, hydrangeas are Al-hyperaccumulators and exhibit tolerance to acidic soils, in which the toxicity is due to soluble Al ion. Therefore, an Al-absorbing transport and storage system must exist in hydrangea. Recently, we cloned vacuolar and plasma membrane-localized Al-transporters, HmVALT, and HmPALT1, which are both members of the aquaporin family. However, HmPALT1 was only expressed in the sepals, indicating that a different Al-transporter should exist for absorption and long-distance transportation in the hydrangea plant. Using genetic information and microarray analysis, we identified an additional aluminum transporter gene, HmPALT2, which belongs to a member of the anion permease. The transcript was expressed in all tissues of hydrangea plants, and a transient expression study indicated that the gene product is localized to the plasma membrane. The results of an aluminum tolerance assay using yeast cells showed that the HmPALT2 is also involved in the transport of other metal(loid)s. The over-expression of HmPALT2 in Arabidopsis resulted in aluminum-hypersensitivity, suggesting that HmPALT2 should work as an aluminum transporter into cells in planta.",
author = "Takashi Negishi and Kenshiro Oshima and Masahira Hattori and Kumi Yoshida",
year = "2013",
month = "5",
doi = "10.1111/gtc.12041",
language = "English",
volume = "18",
pages = "341--352",
journal = "Genes to Cells",
issn = "1356-9597",
publisher = "Wiley-Blackwell",
number = "5",

}

TY - JOUR

T1 - Plasma membrane-localized Al-transporter from blue hydrangea sepals is a member of the anion permease family

AU - Negishi, Takashi

AU - Oshima, Kenshiro

AU - Hattori, Masahira

AU - Yoshida, Kumi

PY - 2013/5

Y1 - 2013/5

N2 - In hydrangea sepals, an aluminum complex of delphinidin-3-O-glucoside is responsible for the development of the blue color, and co-existing copigments mediate the solubilization and stabilization of the blue Al-anthocyanin complex which is localized in the sepal vacuole. In addition, hydrangeas are Al-hyperaccumulators and exhibit tolerance to acidic soils, in which the toxicity is due to soluble Al ion. Therefore, an Al-absorbing transport and storage system must exist in hydrangea. Recently, we cloned vacuolar and plasma membrane-localized Al-transporters, HmVALT, and HmPALT1, which are both members of the aquaporin family. However, HmPALT1 was only expressed in the sepals, indicating that a different Al-transporter should exist for absorption and long-distance transportation in the hydrangea plant. Using genetic information and microarray analysis, we identified an additional aluminum transporter gene, HmPALT2, which belongs to a member of the anion permease. The transcript was expressed in all tissues of hydrangea plants, and a transient expression study indicated that the gene product is localized to the plasma membrane. The results of an aluminum tolerance assay using yeast cells showed that the HmPALT2 is also involved in the transport of other metal(loid)s. The over-expression of HmPALT2 in Arabidopsis resulted in aluminum-hypersensitivity, suggesting that HmPALT2 should work as an aluminum transporter into cells in planta.

AB - In hydrangea sepals, an aluminum complex of delphinidin-3-O-glucoside is responsible for the development of the blue color, and co-existing copigments mediate the solubilization and stabilization of the blue Al-anthocyanin complex which is localized in the sepal vacuole. In addition, hydrangeas are Al-hyperaccumulators and exhibit tolerance to acidic soils, in which the toxicity is due to soluble Al ion. Therefore, an Al-absorbing transport and storage system must exist in hydrangea. Recently, we cloned vacuolar and plasma membrane-localized Al-transporters, HmVALT, and HmPALT1, which are both members of the aquaporin family. However, HmPALT1 was only expressed in the sepals, indicating that a different Al-transporter should exist for absorption and long-distance transportation in the hydrangea plant. Using genetic information and microarray analysis, we identified an additional aluminum transporter gene, HmPALT2, which belongs to a member of the anion permease. The transcript was expressed in all tissues of hydrangea plants, and a transient expression study indicated that the gene product is localized to the plasma membrane. The results of an aluminum tolerance assay using yeast cells showed that the HmPALT2 is also involved in the transport of other metal(loid)s. The over-expression of HmPALT2 in Arabidopsis resulted in aluminum-hypersensitivity, suggesting that HmPALT2 should work as an aluminum transporter into cells in planta.

UR - http://www.scopus.com/inward/record.url?scp=84876973066&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=84876973066&partnerID=8YFLogxK

U2 - 10.1111/gtc.12041

DO - 10.1111/gtc.12041

M3 - Article

C2 - 23433438

AN - SCOPUS:84876973066

VL - 18

SP - 341

EP - 352

JO - Genes to Cells

JF - Genes to Cells

SN - 1356-9597

IS - 5

ER -