Platelet-derived growth factor oncoprotein detection using three-dimensional carbon microarrays

Varun Penmatsa, A. Rahim Ruslinda, Majid Beidaghi, Hiroshi Kawarada, Chunlei Wang

    Research output: Contribution to journalArticle

    26 Citations (Scopus)

    Abstract

    The potential of aptamers as ligand binding molecule has opened new avenues in the development of biosensors for cancer oncoproteins. In this paper, a label-free detection strategy using signaling aptamer/protein binding complex for platelet-derived growth factor (PDGF-BB) oncoprotein detection is reported. The detection mechanism is based on the release of fluorophore (TOTO intercalating dye) from the target binding aptamer's stem structure when it captures PDGF. Amino-terminated three-dimensional carbon microarrays fabricated by pyrolyzing patterned photoresist were used as a detection platform. The sensor showed near linear relationship between the relative fluorescence difference and protein concentration even in the sub-nanomolar range with an excellent detection limit of 5. pmol. This detection strategy is promising in a wide range of applications in the detection of cancer biomarkers and other proteins.

    Original languageEnglish
    Pages (from-to)118-123
    Number of pages6
    JournalBiosensors and Bioelectronics
    Volume39
    Issue number1
    DOIs
    Publication statusPublished - 2013 Jan 15

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    Keywords

    • 3D carbon microarrays
    • Direct amination
    • Fluorescence detection
    • Platelet-derived growth factor

    ASJC Scopus subject areas

    • Biophysics
    • Biomedical Engineering
    • Biotechnology
    • Electrochemistry

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