Platelet-Derived Stromal Cell-Derived Factor-1 Is Required for the Transformation of Circulating Monocytes into Multipotential Cells

Noriyuki Seta, Yuka Okazaki, Hiroshi Miyazaki, Takashi Kato, Masataka Kuwana

    Research output: Contribution to journalArticle

    13 Citations (Scopus)

    Abstract

    Background:We previously described a primitive cell population derived from human circulating CD14+ monocytes, named monocyte-derived multipotential cells (MOMCs), which are capable of differentiating into mesenchymal and endothelial lineages. To generate MOMCs in vitro, monocytes are required to bind to fibronectin and be exposed to soluble factor(s) derived from circulating CD14- cells. The present study was conducted to identify factors that induce MOMC differentiation.Methods:We cultured CD14+ monocytes on fibronectin in the presence or absence of platelets, CD14- peripheral blood mononuclear cells, platelet-conditioned medium, or candidate MOMC differentiation factors. The transformation of monocytes into MOMCs was assessed by the presence of spindle-shaped adherent cells, CD34 expression, and the potential to differentiate in vitro into mesenchymal and endothelial lineages.Results:The presence of platelets or platelet-conditioned medium was required to generate MOMCs from monocytes. A screening of candidate platelet-derived soluble factors identified stromal cell-derived factor (SDF)-1 as a requirement for generating MOMCs. Blocking an interaction between SDF-1 and its receptor CXCR4 inhibited MOMC generation, further confirming SDF-1′s critical role in this process. Finally, circulating MOMC precursors were found to reside in the CD14+CXCR4high cell population.Conclusion:The interaction of SDF-1 with CXCR4 is essential for the transformation of circulating monocytes into MOMCs.

    Original languageEnglish
    Article numbere74246
    JournalPLoS One
    Volume8
    Issue number9
    DOIs
    Publication statusPublished - 2013 Sep 16

    Fingerprint

    Chemokine CXCL12
    Platelets
    monocytes
    Monocytes
    Blood Platelets
    Conditioned Culture Medium
    Fibronectins
    cells
    Cells
    CXCR4 Receptors
    Screening
    Blood
    chemokine CXCL12
    fibronectins
    cell differentiation
    Cell Differentiation
    stromal cells

    ASJC Scopus subject areas

    • Agricultural and Biological Sciences(all)
    • Biochemistry, Genetics and Molecular Biology(all)
    • Medicine(all)

    Cite this

    Platelet-Derived Stromal Cell-Derived Factor-1 Is Required for the Transformation of Circulating Monocytes into Multipotential Cells. / Seta, Noriyuki; Okazaki, Yuka; Miyazaki, Hiroshi; Kato, Takashi; Kuwana, Masataka.

    In: PLoS One, Vol. 8, No. 9, e74246, 16.09.2013.

    Research output: Contribution to journalArticle

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