PLC β4-independent Ca2+ rise via muscarinic receptors in the mouse suprachiasmatic nucleus

Masayuki Ikeda, Takashi Sugiyama, Kaoru Suzuki, Takahiro Moriya, Shigenobu Shibata, Motoya Katsuki, Charles N. Allen, Tohru Yoshioka

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13 Citations (Scopus)

Abstract

Cholinergic regulation of the suprachiasmatic nucleus (SCN) has been extensively studied although the intracellular signaling mechanisms are not well understood. We examined immunostaining for phospholipase C-β (PLC-β) families that couple to muscarinic acetylcholine receptors (mAChR) and demonstrated the expression of PLC-βI and β4 in the mouse SCN. Ca2+ imaging analysis indicated that the MI-mAChR antagonist, pirenzepine blocked carbachol-induced Ca2+ elevation in the SCN and the response was equivalent between the wild type and the PLC-β4-knockout mice. In addition, the knockout mice displayed locomotor and temperature rhythms coupling to 24 h light/dark cycles. Therefore, it was proposed that PLC-βI but not PLC-β4 was involved in the mAChR-mediated Ca2+ signaling in the SCN. (C) 2000 Lippincott Williams and Wilkins.

Original languageEnglish
Pages (from-to)907-912
Number of pages6
JournalNeuroReport
Volume11
Issue number5
DOIs
Publication statusPublished - 2000 Apr 7

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Keywords

  • Body temperature
  • Calcium imaging
  • Carbachol
  • Circadian rhythm
  • Immunostaining
  • Locomotor activity
  • Muscarinic acetylcholine receptor
  • Phospholipase C-β
  • Pirenzepine
  • Suprachiasmatic nucleus

ASJC Scopus subject areas

  • Neuroscience(all)

Cite this

Ikeda, M., Sugiyama, T., Suzuki, K., Moriya, T., Shibata, S., Katsuki, M., Allen, C. N., & Yoshioka, T. (2000). PLC β4-independent Ca2+ rise via muscarinic receptors in the mouse suprachiasmatic nucleus. NeuroReport, 11(5), 907-912. https://doi.org/10.1097/00001756-200004070-00002