PLC β4-independent Ca2+ rise via muscarinic receptors in the mouse suprachiasmatic nucleus

Masayuki Ikeda, Takashi Sugiyama, Kaoru Suzuki, Takahiro Moriya, Shigenobu Shibata, Motoya Katsuki, Charles N. Allen, Tohru Yoshioka

    Research output: Contribution to journalArticle

    13 Citations (Scopus)

    Abstract

    Cholinergic regulation of the suprachiasmatic nucleus (SCN) has been extensively studied although the intracellular signaling mechanisms are not well understood. We examined immunostaining for phospholipase C-β (PLC-β) families that couple to muscarinic acetylcholine receptors (mAChR) and demonstrated the expression of PLC-βI and β4 in the mouse SCN. Ca2+ imaging analysis indicated that the MI-mAChR antagonist, pirenzepine blocked carbachol-induced Ca2+ elevation in the SCN and the response was equivalent between the wild type and the PLC-β4-knockout mice. In addition, the knockout mice displayed locomotor and temperature rhythms coupling to 24 h light/dark cycles. Therefore, it was proposed that PLC-βI but not PLC-β4 was involved in the mAChR-mediated Ca2+ signaling in the SCN. (C) 2000 Lippincott Williams and Wilkins.

    Original languageEnglish
    Pages (from-to)907-912
    Number of pages6
    JournalNeuroReport
    Volume11
    Issue number5
    Publication statusPublished - 2000 Apr 7

    Fingerprint

    Suprachiasmatic Nucleus
    Type C Phospholipases
    Muscarinic Receptors
    Knockout Mice
    Pirenzepine
    Photoperiod
    Cholinergic Antagonists
    Carbachol
    Cholinergic Agents
    Temperature

    Keywords

    • Body temperature
    • Calcium imaging
    • Carbachol
    • Circadian rhythm
    • Immunostaining
    • Locomotor activity
    • Muscarinic acetylcholine receptor
    • Phospholipase C-β
    • Pirenzepine
    • Suprachiasmatic nucleus

    ASJC Scopus subject areas

    • Neuroscience(all)

    Cite this

    Ikeda, M., Sugiyama, T., Suzuki, K., Moriya, T., Shibata, S., Katsuki, M., ... Yoshioka, T. (2000). PLC β4-independent Ca2+ rise via muscarinic receptors in the mouse suprachiasmatic nucleus. NeuroReport, 11(5), 907-912.

    PLC β4-independent Ca2+ rise via muscarinic receptors in the mouse suprachiasmatic nucleus. / Ikeda, Masayuki; Sugiyama, Takashi; Suzuki, Kaoru; Moriya, Takahiro; Shibata, Shigenobu; Katsuki, Motoya; Allen, Charles N.; Yoshioka, Tohru.

    In: NeuroReport, Vol. 11, No. 5, 07.04.2000, p. 907-912.

    Research output: Contribution to journalArticle

    Ikeda, M, Sugiyama, T, Suzuki, K, Moriya, T, Shibata, S, Katsuki, M, Allen, CN & Yoshioka, T 2000, 'PLC β4-independent Ca2+ rise via muscarinic receptors in the mouse suprachiasmatic nucleus', NeuroReport, vol. 11, no. 5, pp. 907-912.
    Ikeda M, Sugiyama T, Suzuki K, Moriya T, Shibata S, Katsuki M et al. PLC β4-independent Ca2+ rise via muscarinic receptors in the mouse suprachiasmatic nucleus. NeuroReport. 2000 Apr 7;11(5):907-912.
    Ikeda, Masayuki ; Sugiyama, Takashi ; Suzuki, Kaoru ; Moriya, Takahiro ; Shibata, Shigenobu ; Katsuki, Motoya ; Allen, Charles N. ; Yoshioka, Tohru. / PLC β4-independent Ca2+ rise via muscarinic receptors in the mouse suprachiasmatic nucleus. In: NeuroReport. 2000 ; Vol. 11, No. 5. pp. 907-912.
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