PLC β4-independent Ca2+ rise via muscarinic receptors in the mouse suprachiasmatic nucleus

Masayuki Ikeda; Takashi Sugiyama; Kaoru Suzuki; Takahiro Moriya; Shigenobu Shibata; Motoya Katsuki; Charles N. Allen; Tohru Yoshioka

doi:10.1097/00001756-200004070-00002

PLC β4-independent Ca²+ rise via muscarinic receptors in the mouse suprachiasmatic nucleus

Masayuki Ikeda^*, Takashi Sugiyama, Kaoru Suzuki, Takahiro Moriya, Shigenobu Shibata, Motoya Katsuki, Charles N. Allen, Tohru Yoshioka

^*Corresponding author for this work

School of Advanced Science and Engineering

Research output: Contribution to journal › Article › peer-review

14 Citations (Scopus)

Abstract

Cholinergic regulation of the suprachiasmatic nucleus (SCN) has been extensively studied although the intracellular signaling mechanisms are not well understood. We examined immunostaining for phospholipase C-β (PLC-β) families that couple to muscarinic acetylcholine receptors (mAChR) and demonstrated the expression of PLC-βI and β4 in the mouse SCN. Ca²⁺ imaging analysis indicated that the MI-mAChR antagonist, pirenzepine blocked carbachol-induced Ca²⁺ elevation in the SCN and the response was equivalent between the wild type and the PLC-β4-knockout mice. In addition, the knockout mice displayed locomotor and temperature rhythms coupling to 24 h light/dark cycles. Therefore, it was proposed that PLC-βI but not PLC-β4 was involved in the mAChR-mediated Ca²⁺ signaling in the SCN. (C) 2000 Lippincott Williams and Wilkins.

Original language	English
Pages (from-to)	907-912
Number of pages	6
Journal	NeuroReport
Volume	11
Issue number	5
DOIs	https://doi.org/10.1097/00001756-200004070-00002
Publication status	Published - 2000 Apr 7

Keywords

Body temperature
Calcium imaging
Carbachol
Circadian rhythm
Immunostaining
Locomotor activity
Muscarinic acetylcholine receptor
Phospholipase C-β
Pirenzepine
Suprachiasmatic nucleus

ASJC Scopus subject areas

Neuroscience(all)

Access to Document

10.1097/00001756-200004070-00002

Cite this

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title = "PLC β4-independent Ca2+ rise via muscarinic receptors in the mouse suprachiasmatic nucleus",

abstract = "Cholinergic regulation of the suprachiasmatic nucleus (SCN) has been extensively studied although the intracellular signaling mechanisms are not well understood. We examined immunostaining for phospholipase C-β (PLC-β) families that couple to muscarinic acetylcholine receptors (mAChR) and demonstrated the expression of PLC-βI and β4 in the mouse SCN. Ca2+ imaging analysis indicated that the MI-mAChR antagonist, pirenzepine blocked carbachol-induced Ca2+ elevation in the SCN and the response was equivalent between the wild type and the PLC-β4-knockout mice. In addition, the knockout mice displayed locomotor and temperature rhythms coupling to 24 h light/dark cycles. Therefore, it was proposed that PLC-βI but not PLC-β4 was involved in the mAChR-mediated Ca2+ signaling in the SCN. (C) 2000 Lippincott Williams and Wilkins.",

keywords = "Body temperature, Calcium imaging, Carbachol, Circadian rhythm, Immunostaining, Locomotor activity, Muscarinic acetylcholine receptor, Phospholipase C-β, Pirenzepine, Suprachiasmatic nucleus",

author = "Masayuki Ikeda and Takashi Sugiyama and Kaoru Suzuki and Takahiro Moriya and Shigenobu Shibata and Motoya Katsuki and Allen, {Charles N.} and Tohru Yoshioka",

year = "2000",

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T1 - PLC β4-independent Ca2+ rise via muscarinic receptors in the mouse suprachiasmatic nucleus

AU - Ikeda, Masayuki

AU - Sugiyama, Takashi

AU - Suzuki, Kaoru

AU - Moriya, Takahiro

AU - Shibata, Shigenobu

AU - Katsuki, Motoya

AU - Allen, Charles N.

AU - Yoshioka, Tohru

PY - 2000/4/7

Y1 - 2000/4/7

N2 - Cholinergic regulation of the suprachiasmatic nucleus (SCN) has been extensively studied although the intracellular signaling mechanisms are not well understood. We examined immunostaining for phospholipase C-β (PLC-β) families that couple to muscarinic acetylcholine receptors (mAChR) and demonstrated the expression of PLC-βI and β4 in the mouse SCN. Ca2+ imaging analysis indicated that the MI-mAChR antagonist, pirenzepine blocked carbachol-induced Ca2+ elevation in the SCN and the response was equivalent between the wild type and the PLC-β4-knockout mice. In addition, the knockout mice displayed locomotor and temperature rhythms coupling to 24 h light/dark cycles. Therefore, it was proposed that PLC-βI but not PLC-β4 was involved in the mAChR-mediated Ca2+ signaling in the SCN. (C) 2000 Lippincott Williams and Wilkins.

AB - Cholinergic regulation of the suprachiasmatic nucleus (SCN) has been extensively studied although the intracellular signaling mechanisms are not well understood. We examined immunostaining for phospholipase C-β (PLC-β) families that couple to muscarinic acetylcholine receptors (mAChR) and demonstrated the expression of PLC-βI and β4 in the mouse SCN. Ca2+ imaging analysis indicated that the MI-mAChR antagonist, pirenzepine blocked carbachol-induced Ca2+ elevation in the SCN and the response was equivalent between the wild type and the PLC-β4-knockout mice. In addition, the knockout mice displayed locomotor and temperature rhythms coupling to 24 h light/dark cycles. Therefore, it was proposed that PLC-βI but not PLC-β4 was involved in the mAChR-mediated Ca2+ signaling in the SCN. (C) 2000 Lippincott Williams and Wilkins.

KW - Body temperature

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KW - Immunostaining

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KW - Muscarinic acetylcholine receptor

KW - Phospholipase C-β

KW - Pirenzepine

KW - Suprachiasmatic nucleus

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