TY - JOUR
T1 - Polycomb group protein Ezh1 represses Nodal and maintains the left-right axis
AU - Arai, Daisuke
AU - Katsura, Hiroaki
AU - Shindo, Norihisa
AU - Matsumoto, Mayuko
AU - Higashinakagawa, Toru
N1 - Funding Information:
We thank T. Czerny, H. Takeda, and D. Araki for cDNA probes, and H. Hamada for the FoxH1 expression vector. This work was supported by a Grant-in-Aid for JSPS Fellows (09329300) to D. Arai.
PY - 2010/5
Y1 - 2010/5
N2 - The left-right (LR) axis is essential for the proper function of internal organs. In mammals and fish, left-sided Nodal expression governs LR patterning. Here, we show that the Polycomb group protein Ezh1, which is highly conserved from fish to human, participates in LR patterning. Knockdown of olezh1, a medaka homologue of Ezh1, led to LR reversal of internal organs. It was shown that OLEZH1 acts in silencing the expression of Spaw (a medaka homolog of Nodal) via a previously unknown pathway. Furthermore, coimmunoprecipitation showed physical interaction of Ezh1 with FoxH1, a Nodal regulator. This represents a novel mechanism for LR patterning and implies that Ezh1 has developmental importance.
AB - The left-right (LR) axis is essential for the proper function of internal organs. In mammals and fish, left-sided Nodal expression governs LR patterning. Here, we show that the Polycomb group protein Ezh1, which is highly conserved from fish to human, participates in LR patterning. Knockdown of olezh1, a medaka homologue of Ezh1, led to LR reversal of internal organs. It was shown that OLEZH1 acts in silencing the expression of Spaw (a medaka homolog of Nodal) via a previously unknown pathway. Furthermore, coimmunoprecipitation showed physical interaction of Ezh1 with FoxH1, a Nodal regulator. This represents a novel mechanism for LR patterning and implies that Ezh1 has developmental importance.
KW - Ezh1
KW - Left-right axis
KW - Medaka
KW - Nodal
KW - Polycomb
UR - http://www.scopus.com/inward/record.url?scp=77952318367&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=77952318367&partnerID=8YFLogxK
U2 - 10.1016/j.ydbio.2010.03.002
DO - 10.1016/j.ydbio.2010.03.002
M3 - Article
C2 - 20227405
AN - SCOPUS:77952318367
VL - 341
SP - 459
EP - 463
JO - Developmental Biology
JF - Developmental Biology
SN - 0012-1606
IS - 2
ER -