Polycomb group protein Ezh1 represses Nodal and maintains the left-right axis

Daisuke Arai; Hiroaki Katsura; Norihisa Shindo; Mayuko Matsumoto; Toru Higashinakagawa

doi:10.1016/j.ydbio.2010.03.002

Polycomb group protein Ezh1 represses Nodal and maintains the left-right axis

Daisuke Arai, Hiroaki Katsura, Norihisa Shindo, Mayuko Matsumoto, Toru Higashinakagawa

School of Advanced Science and Engineering

Research output: Contribution to journal › Article › peer-review

6 Citations (Scopus)

Abstract

The left-right (LR) axis is essential for the proper function of internal organs. In mammals and fish, left-sided Nodal expression governs LR patterning. Here, we show that the Polycomb group protein Ezh1, which is highly conserved from fish to human, participates in LR patterning. Knockdown of olezh1, a medaka homologue of Ezh1, led to LR reversal of internal organs. It was shown that OLEZH1 acts in silencing the expression of Spaw (a medaka homolog of Nodal) via a previously unknown pathway. Furthermore, coimmunoprecipitation showed physical interaction of Ezh1 with FoxH1, a Nodal regulator. This represents a novel mechanism for LR patterning and implies that Ezh1 has developmental importance.

Original language	English
Pages (from-to)	459-463
Number of pages	5
Journal	Developmental Biology
Volume	341
Issue number	2
DOIs	https://doi.org/10.1016/j.ydbio.2010.03.002
Publication status	Published - 2010 May

Keywords

Ezh1
Left-right axis
Medaka
Nodal
Polycomb

ASJC Scopus subject areas

Molecular Biology
Developmental Biology
Cell Biology

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title = "Polycomb group protein Ezh1 represses Nodal and maintains the left-right axis",

abstract = "The left-right (LR) axis is essential for the proper function of internal organs. In mammals and fish, left-sided Nodal expression governs LR patterning. Here, we show that the Polycomb group protein Ezh1, which is highly conserved from fish to human, participates in LR patterning. Knockdown of olezh1, a medaka homologue of Ezh1, led to LR reversal of internal organs. It was shown that OLEZH1 acts in silencing the expression of Spaw (a medaka homolog of Nodal) via a previously unknown pathway. Furthermore, coimmunoprecipitation showed physical interaction of Ezh1 with FoxH1, a Nodal regulator. This represents a novel mechanism for LR patterning and implies that Ezh1 has developmental importance.",

keywords = "Ezh1, Left-right axis, Medaka, Nodal, Polycomb",

author = "Daisuke Arai and Hiroaki Katsura and Norihisa Shindo and Mayuko Matsumoto and Toru Higashinakagawa",

note = "Funding Information: We thank T. Czerny, H. Takeda, and D. Araki for cDNA probes, and H. Hamada for the FoxH1 expression vector. This work was supported by a Grant-in-Aid for JSPS Fellows (09329300) to D. Arai. ",

year = "2010",

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T1 - Polycomb group protein Ezh1 represses Nodal and maintains the left-right axis

AU - Arai, Daisuke

AU - Katsura, Hiroaki

AU - Shindo, Norihisa

AU - Matsumoto, Mayuko

AU - Higashinakagawa, Toru

N1 - Funding Information: We thank T. Czerny, H. Takeda, and D. Araki for cDNA probes, and H. Hamada for the FoxH1 expression vector. This work was supported by a Grant-in-Aid for JSPS Fellows (09329300) to D. Arai.

PY - 2010/5

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N2 - The left-right (LR) axis is essential for the proper function of internal organs. In mammals and fish, left-sided Nodal expression governs LR patterning. Here, we show that the Polycomb group protein Ezh1, which is highly conserved from fish to human, participates in LR patterning. Knockdown of olezh1, a medaka homologue of Ezh1, led to LR reversal of internal organs. It was shown that OLEZH1 acts in silencing the expression of Spaw (a medaka homolog of Nodal) via a previously unknown pathway. Furthermore, coimmunoprecipitation showed physical interaction of Ezh1 with FoxH1, a Nodal regulator. This represents a novel mechanism for LR patterning and implies that Ezh1 has developmental importance.

AB - The left-right (LR) axis is essential for the proper function of internal organs. In mammals and fish, left-sided Nodal expression governs LR patterning. Here, we show that the Polycomb group protein Ezh1, which is highly conserved from fish to human, participates in LR patterning. Knockdown of olezh1, a medaka homologue of Ezh1, led to LR reversal of internal organs. It was shown that OLEZH1 acts in silencing the expression of Spaw (a medaka homolog of Nodal) via a previously unknown pathway. Furthermore, coimmunoprecipitation showed physical interaction of Ezh1 with FoxH1, a Nodal regulator. This represents a novel mechanism for LR patterning and implies that Ezh1 has developmental importance.

KW - Ezh1

KW - Left-right axis

KW - Medaka

KW - Nodal

KW - Polycomb

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