Abstract
The left-right (LR) axis is essential for the proper function of internal organs. In mammals and fish, left-sided Nodal expression governs LR patterning. Here, we show that the Polycomb group protein Ezh1, which is highly conserved from fish to human, participates in LR patterning. Knockdown of olezh1, a medaka homologue of Ezh1, led to LR reversal of internal organs. It was shown that OLEZH1 acts in silencing the expression of Spaw (a medaka homolog of Nodal) via a previously unknown pathway. Furthermore, coimmunoprecipitation showed physical interaction of Ezh1 with FoxH1, a Nodal regulator. This represents a novel mechanism for LR patterning and implies that Ezh1 has developmental importance.
| Original language | English |
|---|---|
| Pages (from-to) | 459-463 |
| Number of pages | 5 |
| Journal | Developmental Biology |
| Volume | 341 |
| Issue number | 2 |
| DOIs | |
| Publication status | Published - 2010 May |
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Keywords
- Ezh1
- Left-right axis
- Medaka
- Nodal
- Polycomb
ASJC Scopus subject areas
- Developmental Biology
- Cell Biology
- Molecular Biology
Cite this
Polycomb group protein Ezh1 represses Nodal and maintains the left-right axis. / Arai, Daisuke; Katsura, Hiroaki; Shindo, Norihisa; Matsumoto, Mayuko; Higashinakagawa, Toru.
In: Developmental Biology, Vol. 341, No. 2, 05.2010, p. 459-463.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Polycomb group protein Ezh1 represses Nodal and maintains the left-right axis
AU - Arai, Daisuke
AU - Katsura, Hiroaki
AU - Shindo, Norihisa
AU - Matsumoto, Mayuko
AU - Higashinakagawa, Toru
PY - 2010/5
Y1 - 2010/5
N2 - The left-right (LR) axis is essential for the proper function of internal organs. In mammals and fish, left-sided Nodal expression governs LR patterning. Here, we show that the Polycomb group protein Ezh1, which is highly conserved from fish to human, participates in LR patterning. Knockdown of olezh1, a medaka homologue of Ezh1, led to LR reversal of internal organs. It was shown that OLEZH1 acts in silencing the expression of Spaw (a medaka homolog of Nodal) via a previously unknown pathway. Furthermore, coimmunoprecipitation showed physical interaction of Ezh1 with FoxH1, a Nodal regulator. This represents a novel mechanism for LR patterning and implies that Ezh1 has developmental importance.
AB - The left-right (LR) axis is essential for the proper function of internal organs. In mammals and fish, left-sided Nodal expression governs LR patterning. Here, we show that the Polycomb group protein Ezh1, which is highly conserved from fish to human, participates in LR patterning. Knockdown of olezh1, a medaka homologue of Ezh1, led to LR reversal of internal organs. It was shown that OLEZH1 acts in silencing the expression of Spaw (a medaka homolog of Nodal) via a previously unknown pathway. Furthermore, coimmunoprecipitation showed physical interaction of Ezh1 with FoxH1, a Nodal regulator. This represents a novel mechanism for LR patterning and implies that Ezh1 has developmental importance.
KW - Ezh1
KW - Left-right axis
KW - Medaka
KW - Nodal
KW - Polycomb
UR - http://www.scopus.com/inward/record.url?scp=77952318367&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=77952318367&partnerID=8YFLogxK
U2 - 10.1016/j.ydbio.2010.03.002
DO - 10.1016/j.ydbio.2010.03.002
M3 - Article
VL - 341
SP - 459
EP - 463
JO - Developmental Biology
JF - Developmental Biology
SN - 0012-1606
IS - 2
ER -