Polycomb group protein Ezh1 represses Nodal and maintains the left-right axis

Daisuke Arai; Hiroaki Katsura; Norihisa Shindo; Mayuko Matsumoto; Toru Higashinakagawa

doi:10.1016/j.ydbio.2010.03.002

Polycomb group protein Ezh1 represses Nodal and maintains the left-right axis

Daisuke Arai, Hiroaki Katsura, Norihisa Shindo, Mayuko Matsumoto, Toru Higashinakagawa

Waseda Research Institute for Science and Engineering

Research output: Contribution to journal › Article

6 Citations (Scopus)

Abstract

The left-right (LR) axis is essential for the proper function of internal organs. In mammals and fish, left-sided Nodal expression governs LR patterning. Here, we show that the Polycomb group protein Ezh1, which is highly conserved from fish to human, participates in LR patterning. Knockdown of olezh1, a medaka homologue of Ezh1, led to LR reversal of internal organs. It was shown that OLEZH1 acts in silencing the expression of Spaw (a medaka homolog of Nodal) via a previously unknown pathway. Furthermore, coimmunoprecipitation showed physical interaction of Ezh1 with FoxH1, a Nodal regulator. This represents a novel mechanism for LR patterning and implies that Ezh1 has developmental importance.

Original language	English
Pages (from-to)	459-463
Number of pages	5
Journal	Developmental Biology
Volume	341
Issue number	2
DOIs	https://doi.org/10.1016/j.ydbio.2010.03.002
Publication status	Published - 2010 May

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Keywords

Ezh1
Left-right axis
Medaka
Nodal
Polycomb

ASJC Scopus subject areas

Developmental Biology
Cell Biology
Molecular Biology

Cite this

Arai, D., Katsura, H., Shindo, N., Matsumoto, M., & Higashinakagawa, T. (2010). Polycomb group protein Ezh1 represses Nodal and maintains the left-right axis. Developmental Biology, 341(2), 459-463. https://doi.org/10.1016/j.ydbio.2010.03.002

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abstract = "The left-right (LR) axis is essential for the proper function of internal organs. In mammals and fish, left-sided Nodal expression governs LR patterning. Here, we show that the Polycomb group protein Ezh1, which is highly conserved from fish to human, participates in LR patterning. Knockdown of olezh1, a medaka homologue of Ezh1, led to LR reversal of internal organs. It was shown that OLEZH1 acts in silencing the expression of Spaw (a medaka homolog of Nodal) via a previously unknown pathway. Furthermore, coimmunoprecipitation showed physical interaction of Ezh1 with FoxH1, a Nodal regulator. This represents a novel mechanism for LR patterning and implies that Ezh1 has developmental importance.",

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10.1016/j.ydbio.2010.03.002