Poly(ethylene glycol)-modification of the phospholipid vesicles by using the spontaneous incorporation of poly(ethylene glycol)-lipid into the vesicles

Keitaro Sou, Taro Endo, Shinji Takeoka, Eishun Tsuchida

    Research output: Contribution to journalArticle

    116 Citations (Scopus)

    Abstract

    The critical micelle concentrations of 1,2-dipalmitoyl-sn-glycero-3-phosphoethanolamine-N-[monomethoxy poly(ethylene glycol) (5000)] (PEG-DPPE) and its distearoyl analogue (PEG-DSPE) were 70 and 9 μM, respectively, in buffer solutions ([Tris] = 20 mM, [NaCl] = 140 mM, pH 7.4) at 37 °C. When these PEG-lipid micelle dispersions were mixed with the dispersions of phospholipid vesicles comprised of a C16 membrane, of which the carbon number is 16, or a C18 membrane, the PEG-lipid micelles were dissociated into monomers and then spontaneously incorporated into the surface of the preformed vesicles. The incorporation rates and the enthalpy changes during incorporation were measured with an isothermal titration microcalorimeter. The incorporation rate of PEG-DPPE was faster than that of PEG-DSPE, because the dissociation rate of the PEG-DPPE micelles was faster than that of PEG-DSPE micelles. The incorporation equilibrium constant of PEG-DSPE was larger than that of PEG-DPPE due to its slow dissociation rate from the membrane, caused by the stronger hydrophobic interaction. The combination of PEG-DSPE and the C18 membrane was the most thermodynamically stabilized pair. Furthermore, the dispersion stability of the surface-modified vesicles prepared by this spontaneous incorporation was analyzed by using the critical molecular weight of the polymer for the aggregation of vesicles. The aggregation of the vesicles was successfully supressed with an increase in the molecular weight of the PEG in the PEG-lipid and its incorporation ratio.

    Original languageEnglish
    Pages (from-to)372-379
    Number of pages8
    JournalBioconjugate Chemistry
    Volume11
    Issue number3
    DOIs
    Publication statusPublished - 2000 May

    Fingerprint

    Ethylene Glycol
    Phospholipids
    Micelles
    Lipids
    Polyethylene glycols
    Membranes
    Dispersions
    Agglomeration
    Molecular Weight
    Molecular weight
    Tromethamine
    Critical micelle concentration
    Equilibrium constants
    Membrane Lipids
    Titration
    Hydrophobic and Hydrophilic Interactions
    Enthalpy
    Buffers
    Polymers
    Carbon

    ASJC Scopus subject areas

    • Chemistry(all)
    • Organic Chemistry
    • Clinical Biochemistry
    • Biochemistry, Genetics and Molecular Biology(all)
    • Biochemistry

    Cite this

    Poly(ethylene glycol)-modification of the phospholipid vesicles by using the spontaneous incorporation of poly(ethylene glycol)-lipid into the vesicles. / Sou, Keitaro; Endo, Taro; Takeoka, Shinji; Tsuchida, Eishun.

    In: Bioconjugate Chemistry, Vol. 11, No. 3, 05.2000, p. 372-379.

    Research output: Contribution to journalArticle

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