Polylactic acid nanosheets in prevention of postoperative intestinal adhesion and their effects on bacterial propagation in an experimental model

A. Hinoki, A. Saito, M. Kinoshita, J. Yamamoto, D. Saitoh, Shinji Takeoka

    Research output: Contribution to journalArticle

    6 Citations (Scopus)

    Abstract

    Background Ultrathin films (nanosheets) adhere tightly to organ surfaces but prevent adhesion to other organs. The antiadhesive effect of nanosheets and their effect on bacterial propagation were investigated in a murine intestinal adhesion model. Methods Polylactic acid nanosheets (approximately 80 nm thick) were produced. Serosal defects were created by peeling off the intestinal serosa; these were left open or covered with nanosheets or Seprafilm® and the formation of intestinal adhesions was analysed. To examine bacterial propagation, a nanosheet or Seprafilm® was placed on intact murine jejunum followed by Escherichia coli inoculation at the site. Results Treatment both with nanosheets and with Seprafilm® reduced postoperative intestinal adhesion (mean adhesion score 0·67 for nanosheets, 0·43 for Seprafilm® and 2·87 for no antiadhesive treatment; P <0·001 for nanosheets or Seprafilm® versus no adhesive treatment). Nanosheet treatment did not affect bacterial propagation in the peritoneal cavity, whereas Seprafilm®-treated mice showed bacterial propagation, leading to increased mortality. Conclusion Nanosheets may be effective novel antiadhesive agents even in the presence of bacterial contamination. Surgical relevance Intra-abdominal adhesions following surgical contamination can trigger postoperative complications and lead to deterioration in long-term quality of life. However, currently there are no effective antiadhesion materials to prevent the formation of adhesions. Treatment with ultrathin nanosheets effectively reduced postoperative intestinal adhesion in an experimental mouse model, and did not affect bacterial propagation in the peritoneal cavity. These nanosheets are potent novel antiadhesive materials that potentially can be applied even in contaminated conditions.

    Original languageEnglish
    Pages (from-to)692-700
    Number of pages9
    JournalBritish Journal of Surgery
    Volume103
    Issue number6
    DOIs
    Publication statusPublished - 2016 May 1

    Fingerprint

    Theoretical Models
    Peritoneal Cavity
    Tissue Adhesions
    Serous Membrane
    Jejunum
    Adhesives
    Seprafilm
    poly(lactic acid)
    Quality of Life
    Escherichia coli
    Mortality

    ASJC Scopus subject areas

    • Surgery

    Cite this

    Polylactic acid nanosheets in prevention of postoperative intestinal adhesion and their effects on bacterial propagation in an experimental model. / Hinoki, A.; Saito, A.; Kinoshita, M.; Yamamoto, J.; Saitoh, D.; Takeoka, Shinji.

    In: British Journal of Surgery, Vol. 103, No. 6, 01.05.2016, p. 692-700.

    Research output: Contribution to journalArticle

    @article{f566a0e3c6284a80bf73c48d0fe16319,
    title = "Polylactic acid nanosheets in prevention of postoperative intestinal adhesion and their effects on bacterial propagation in an experimental model",
    abstract = "Background Ultrathin films (nanosheets) adhere tightly to organ surfaces but prevent adhesion to other organs. The antiadhesive effect of nanosheets and their effect on bacterial propagation were investigated in a murine intestinal adhesion model. Methods Polylactic acid nanosheets (approximately 80 nm thick) were produced. Serosal defects were created by peeling off the intestinal serosa; these were left open or covered with nanosheets or Seprafilm{\circledR} and the formation of intestinal adhesions was analysed. To examine bacterial propagation, a nanosheet or Seprafilm{\circledR} was placed on intact murine jejunum followed by Escherichia coli inoculation at the site. Results Treatment both with nanosheets and with Seprafilm{\circledR} reduced postoperative intestinal adhesion (mean adhesion score 0·67 for nanosheets, 0·43 for Seprafilm{\circledR} and 2·87 for no antiadhesive treatment; P <0·001 for nanosheets or Seprafilm{\circledR} versus no adhesive treatment). Nanosheet treatment did not affect bacterial propagation in the peritoneal cavity, whereas Seprafilm{\circledR}-treated mice showed bacterial propagation, leading to increased mortality. Conclusion Nanosheets may be effective novel antiadhesive agents even in the presence of bacterial contamination. Surgical relevance Intra-abdominal adhesions following surgical contamination can trigger postoperative complications and lead to deterioration in long-term quality of life. However, currently there are no effective antiadhesion materials to prevent the formation of adhesions. Treatment with ultrathin nanosheets effectively reduced postoperative intestinal adhesion in an experimental mouse model, and did not affect bacterial propagation in the peritoneal cavity. These nanosheets are potent novel antiadhesive materials that potentially can be applied even in contaminated conditions.",
    author = "A. Hinoki and A. Saito and M. Kinoshita and J. Yamamoto and D. Saitoh and Shinji Takeoka",
    year = "2016",
    month = "5",
    day = "1",
    doi = "10.1002/bjs.10122",
    language = "English",
    volume = "103",
    pages = "692--700",
    journal = "British Journal of Surgery",
    issn = "0007-1323",
    publisher = "John Wiley and Sons Ltd",
    number = "6",

    }

    TY - JOUR

    T1 - Polylactic acid nanosheets in prevention of postoperative intestinal adhesion and their effects on bacterial propagation in an experimental model

    AU - Hinoki, A.

    AU - Saito, A.

    AU - Kinoshita, M.

    AU - Yamamoto, J.

    AU - Saitoh, D.

    AU - Takeoka, Shinji

    PY - 2016/5/1

    Y1 - 2016/5/1

    N2 - Background Ultrathin films (nanosheets) adhere tightly to organ surfaces but prevent adhesion to other organs. The antiadhesive effect of nanosheets and their effect on bacterial propagation were investigated in a murine intestinal adhesion model. Methods Polylactic acid nanosheets (approximately 80 nm thick) were produced. Serosal defects were created by peeling off the intestinal serosa; these were left open or covered with nanosheets or Seprafilm® and the formation of intestinal adhesions was analysed. To examine bacterial propagation, a nanosheet or Seprafilm® was placed on intact murine jejunum followed by Escherichia coli inoculation at the site. Results Treatment both with nanosheets and with Seprafilm® reduced postoperative intestinal adhesion (mean adhesion score 0·67 for nanosheets, 0·43 for Seprafilm® and 2·87 for no antiadhesive treatment; P <0·001 for nanosheets or Seprafilm® versus no adhesive treatment). Nanosheet treatment did not affect bacterial propagation in the peritoneal cavity, whereas Seprafilm®-treated mice showed bacterial propagation, leading to increased mortality. Conclusion Nanosheets may be effective novel antiadhesive agents even in the presence of bacterial contamination. Surgical relevance Intra-abdominal adhesions following surgical contamination can trigger postoperative complications and lead to deterioration in long-term quality of life. However, currently there are no effective antiadhesion materials to prevent the formation of adhesions. Treatment with ultrathin nanosheets effectively reduced postoperative intestinal adhesion in an experimental mouse model, and did not affect bacterial propagation in the peritoneal cavity. These nanosheets are potent novel antiadhesive materials that potentially can be applied even in contaminated conditions.

    AB - Background Ultrathin films (nanosheets) adhere tightly to organ surfaces but prevent adhesion to other organs. The antiadhesive effect of nanosheets and their effect on bacterial propagation were investigated in a murine intestinal adhesion model. Methods Polylactic acid nanosheets (approximately 80 nm thick) were produced. Serosal defects were created by peeling off the intestinal serosa; these were left open or covered with nanosheets or Seprafilm® and the formation of intestinal adhesions was analysed. To examine bacterial propagation, a nanosheet or Seprafilm® was placed on intact murine jejunum followed by Escherichia coli inoculation at the site. Results Treatment both with nanosheets and with Seprafilm® reduced postoperative intestinal adhesion (mean adhesion score 0·67 for nanosheets, 0·43 for Seprafilm® and 2·87 for no antiadhesive treatment; P <0·001 for nanosheets or Seprafilm® versus no adhesive treatment). Nanosheet treatment did not affect bacterial propagation in the peritoneal cavity, whereas Seprafilm®-treated mice showed bacterial propagation, leading to increased mortality. Conclusion Nanosheets may be effective novel antiadhesive agents even in the presence of bacterial contamination. Surgical relevance Intra-abdominal adhesions following surgical contamination can trigger postoperative complications and lead to deterioration in long-term quality of life. However, currently there are no effective antiadhesion materials to prevent the formation of adhesions. Treatment with ultrathin nanosheets effectively reduced postoperative intestinal adhesion in an experimental mouse model, and did not affect bacterial propagation in the peritoneal cavity. These nanosheets are potent novel antiadhesive materials that potentially can be applied even in contaminated conditions.

    UR - http://www.scopus.com/inward/record.url?scp=84963533324&partnerID=8YFLogxK

    UR - http://www.scopus.com/inward/citedby.url?scp=84963533324&partnerID=8YFLogxK

    U2 - 10.1002/bjs.10122

    DO - 10.1002/bjs.10122

    M3 - Article

    C2 - 26936491

    AN - SCOPUS:84963533324

    VL - 103

    SP - 692

    EP - 700

    JO - British Journal of Surgery

    JF - British Journal of Surgery

    SN - 0007-1323

    IS - 6

    ER -