Polymer microchip integrated with nano-electrospray tip for electrophoresis-mass spectrometry

Hidetoshi Shinohara, Takafumi Suzuki, Fumihiko Kitagawa, Jun Mizuno, Koji Otsuka, Shuichi Shoji

Research output: Contribution to journalArticle

32 Citations (Scopus)

Abstract

A novel cyclo-olefin polymer (COP) microchip was developed integrated with an electrospray ionization (ESI) emitter tip for microchip electrophoretic separation and mass spectrometric detection. The microchip was fabricated by hot embossing and low-temperature direct bonding. The ESI emitter tip structure was formed directly at the microchip electrophoresis (MCE) outlet. Since these microchips enable negligible dead volume at the electrospray port, efficient spray of the sample necessary for acceptable resolution mass spectrometry (MS) was realized. Stable spray was also achieved at the low flow rate (∼0.1 μL/min) without additional pump. By applying the fabricated COP chip to the MCE-ESI-MS analysis, successful separation and detection of caffeine and arginine was achieved.

Original languageEnglish
Pages (from-to)368-373
Number of pages6
JournalSensors and Actuators, B: Chemical
Volume132
Issue number2
DOIs
Publication statusPublished - 2008 Jun 16

Fingerprint

Electrospray ionization
electrophoresis
Electrophoresis
Mass spectrometry
Polymers
mass spectroscopy
Alkenes
ionization
alkenes
Olefins
sprayers
polymers
emitters
Caffeine
caffeine
embossing
Arginine
outlets
flow velocity
chips

Keywords

  • Cyclo-olefin polymer
  • Electrospray ionization
  • Mass spectrometry
  • Microchip electrophoresis

ASJC Scopus subject areas

  • Analytical Chemistry
  • Electrochemistry
  • Electrical and Electronic Engineering

Cite this

Polymer microchip integrated with nano-electrospray tip for electrophoresis-mass spectrometry. / Shinohara, Hidetoshi; Suzuki, Takafumi; Kitagawa, Fumihiko; Mizuno, Jun; Otsuka, Koji; Shoji, Shuichi.

In: Sensors and Actuators, B: Chemical, Vol. 132, No. 2, 16.06.2008, p. 368-373.

Research output: Contribution to journalArticle

Shinohara, Hidetoshi ; Suzuki, Takafumi ; Kitagawa, Fumihiko ; Mizuno, Jun ; Otsuka, Koji ; Shoji, Shuichi. / Polymer microchip integrated with nano-electrospray tip for electrophoresis-mass spectrometry. In: Sensors and Actuators, B: Chemical. 2008 ; Vol. 132, No. 2. pp. 368-373.
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