Polymicrobial Amniotic Fluid Infection with Mycoplasma/Ureaplasma and Other Bacteria Induces Severe Intra-Amniotic Inflammation Associated with Poor Perinatal Prognosis in Preterm Labor

Noriko Yoneda, Satoshi Yoneda, Hideki Niimi, Tomohiro Ueno, Shirou Hayashi, Mika Ito, Arihiro Shiozaki, Daichi Urushiyama, Kenichiro Hata, Wataru Suda, Masahira Hattori, Mika Kigawa, Isao Kitajima, Shigeru Saito

    Research output: Contribution to journalArticle

    23 Citations (Scopus)

    Abstract

    Problem: To study the relationship between perinatal prognosis in cases of preterm labor (PTL) and polymicrobial infection in amniotic fluid (AF) and intra-amniotic (IA) inflammation using a highly sensitive and reliable PCR-based method. Method of Study: To detect prokaryotes using a nested PCR-based method, eukaryote-made thermostable DNA polymerase without bacterial DNA contamination was used in combination with bacterial universal primers. We collected AF aseptically from 118 PTL cases and 50 term subjects. Results: The prevalence of microorganisms was 33% (39/118) by PCR and only 7.6% (9/118) by culture. PTL caused by a combination of positive Mycoplasma/Ureaplasma and other bacteria had significantly higher AF IL-8 levels and a significantly shorter amniocentesis-to-delivery interval. Conclusions: Our newly established PCR method is useful for detecting IA microorganisms. Polymicrobial infection with Mycoplasma/Ureaplasma and other bacteria induces severe IA inflammation associated with poor perinatal prognosis in PTL.

    Original languageEnglish
    Pages (from-to)112-125
    Number of pages14
    JournalAmerican Journal of Reproductive Immunology
    Volume75
    Issue number2
    DOIs
    Publication statusPublished - 2016 Feb 1

    Fingerprint

    Ureaplasma
    Mycoplasma Infections
    Premature Obstetric Labor
    Amniotic Fluid
    Inflammation
    Bacteria
    Polymerase Chain Reaction
    Mycoplasma
    Coinfection
    DNA Contamination
    Bacterial DNA
    Amniocentesis
    DNA-Directed DNA Polymerase
    Eukaryota
    Interleukin-8

    Keywords

    • Eukaryote-made thermostable DNA polymerase
    • Intra-amniotic infection
    • Polymerase chain reaction
    • Polymicrobial infection
    • Preterm labor
    • Ureaplasma spp

    ASJC Scopus subject areas

    • Immunology
    • Immunology and Allergy
    • Obstetrics and Gynaecology
    • Reproductive Medicine

    Cite this

    Polymicrobial Amniotic Fluid Infection with Mycoplasma/Ureaplasma and Other Bacteria Induces Severe Intra-Amniotic Inflammation Associated with Poor Perinatal Prognosis in Preterm Labor. / Yoneda, Noriko; Yoneda, Satoshi; Niimi, Hideki; Ueno, Tomohiro; Hayashi, Shirou; Ito, Mika; Shiozaki, Arihiro; Urushiyama, Daichi; Hata, Kenichiro; Suda, Wataru; Hattori, Masahira; Kigawa, Mika; Kitajima, Isao; Saito, Shigeru.

    In: American Journal of Reproductive Immunology, Vol. 75, No. 2, 01.02.2016, p. 112-125.

    Research output: Contribution to journalArticle

    Yoneda, N, Yoneda, S, Niimi, H, Ueno, T, Hayashi, S, Ito, M, Shiozaki, A, Urushiyama, D, Hata, K, Suda, W, Hattori, M, Kigawa, M, Kitajima, I & Saito, S 2016, 'Polymicrobial Amniotic Fluid Infection with Mycoplasma/Ureaplasma and Other Bacteria Induces Severe Intra-Amniotic Inflammation Associated with Poor Perinatal Prognosis in Preterm Labor', American Journal of Reproductive Immunology, vol. 75, no. 2, pp. 112-125. https://doi.org/10.1111/aji.12456
    Yoneda, Noriko ; Yoneda, Satoshi ; Niimi, Hideki ; Ueno, Tomohiro ; Hayashi, Shirou ; Ito, Mika ; Shiozaki, Arihiro ; Urushiyama, Daichi ; Hata, Kenichiro ; Suda, Wataru ; Hattori, Masahira ; Kigawa, Mika ; Kitajima, Isao ; Saito, Shigeru. / Polymicrobial Amniotic Fluid Infection with Mycoplasma/Ureaplasma and Other Bacteria Induces Severe Intra-Amniotic Inflammation Associated with Poor Perinatal Prognosis in Preterm Labor. In: American Journal of Reproductive Immunology. 2016 ; Vol. 75, No. 2. pp. 112-125.
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