Poly(N-isopropylacrylamide) Carrying Double-Stranded DNA for Affinity Separation of Genotoxins

Daisuke Umeno, Mizuo Maeda

Research output: Contribution to journalArticlepeer-review

24 Citations (Scopus)

Abstract

We immobilized double-stranded DNA on poly(N-isopropylacrylamide), which is temperature-sensitive and gives precipitates from an aqueous solution when heated over 31°C. Photochemical conjugation with a vinyl derivative of psoralen was demonstrated to make the DNA polymerizable with a vinyl monomer. The radical copolymerization with N-isopropylacrylamide gave the temperature-responsive conjugate. The conjugate was shown to capture ethidium, a DNA-binding genotoxin, and precipitate with it when heated. About 95% of ethidium was separated from its highly diluted solution (3 ppm).

Original languageEnglish
Pages (from-to)553-556
Number of pages4
JournalAnalytical Sciences
Volume13
Issue number4
DOIs
Publication statusPublished - 1997
Externally publishedYes

Keywords

  • Affinity separation
  • DNA
  • Ethidium
  • Genotoxin
  • Poly(n-isopropylacrylamide)
  • Precipitation
  • Psoralen

ASJC Scopus subject areas

  • Analytical Chemistry

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