Polyploid formation between Aspergillus niger and Trichoderma viride for enhanced citric acid production from cellulose

Ramida Watanapokasin, Nitisak Sawasjirakij, Shoji Usami, Kotaro Kirimura

    Research output: Contribution to journalArticle

    2 Citations (Scopus)

    Abstract

    The first-stage heterokaryons, obtaining from intergeneric protoplast fusion between Aspergillus niger (Y-b) and Trichoderma viride (M5S51), showed slow growth and mixed morphologies on minimal medium. The fusants were classified into heterokaryon and prototrophic haploid, showing the morphology as that of A. niger. The heterokaryon strains formed conidia with the same nutritional requirements as those of the original auxotrophic mutant strains. After several subcultivations on minimal medium containing d-camphor, some heterokaryon strains formed larger two to seven nuclei/conidium as compared to one nucleus/conidium of the auxotrophic mutant and prototrophic strains, indicating that the new hybrids were generated. Interestingly, three fusant strains AT 11-2-3, AT 11-2-10, and AT 11-2-14 produce 19.2, 6.1, and 10.5 g/l citric acid, respectively, in semisolid culture containing cellulose, whereas A. niger Yang no. 2 could not use carboxymethyl cellulose as the sole carbon source for citric acid production. In addition, the average maximum β-glucosidase and carboxymethylcellulase productions from AT 11-2-3, AT 11-2-10, and AT 11-2-14 were about 16- and 4-folds higher than those of A. niger, respectively.

    Original languageEnglish
    Pages (from-to)176-186
    Number of pages11
    JournalApplied Biochemistry and Biotechnology
    Volume143
    Issue number2
    DOIs
    Publication statusPublished - 2007 Nov

    Fingerprint

    Trichoderma
    Polyploidy
    Aspergillus niger
    Aspergillus
    Citric acid
    Citric Acid
    Cellulose
    Fungal Spores
    Dihydrotachysterol
    Camphor
    Glucosidases
    Nutritional Requirements
    Carboxymethylcellulose Sodium
    Protoplasts
    Haploidy
    Fusion reactions
    Carbon
    Growth

    Keywords

    • Aspergillus niger
    • Citric acid production
    • Intergeneric fusant
    • Polyploid
    • Trichoderma viride

    ASJC Scopus subject areas

    • Biochemistry, Genetics and Molecular Biology(all)
    • Biochemistry
    • Biotechnology
    • Bioengineering

    Cite this

    Polyploid formation between Aspergillus niger and Trichoderma viride for enhanced citric acid production from cellulose. / Watanapokasin, Ramida; Sawasjirakij, Nitisak; Usami, Shoji; Kirimura, Kotaro.

    In: Applied Biochemistry and Biotechnology, Vol. 143, No. 2, 11.2007, p. 176-186.

    Research output: Contribution to journalArticle

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