Polyploid formation between Aspergillus niger and Trichoderma viride for enhanced citric acid production from cellulose

Ramida Watanapokasin, Nitisak Sawasjirakij, Shoji Usami, Kohtaro Kirimura

Research output: Contribution to journalArticle

2 Citations (Scopus)

Abstract

The first-stage heterokaryons, obtaining from intergeneric protoplast fusion between Aspergillus niger (Y-b) and Trichoderma viride (M5S51), showed slow growth and mixed morphologies on minimal medium. The fusants were classified into heterokaryon and prototrophic haploid, showing the morphology as that of A. niger. The heterokaryon strains formed conidia with the same nutritional requirements as those of the original auxotrophic mutant strains. After several subcultivations on minimal medium containing d-camphor, some heterokaryon strains formed larger two to seven nuclei/conidium as compared to one nucleus/conidium of the auxotrophic mutant and prototrophic strains, indicating that the new hybrids were generated. Interestingly, three fusant strains AT 11-2-3, AT 11-2-10, and AT 11-2-14 produce 19.2, 6.1, and 10.5 g/l citric acid, respectively, in semisolid culture containing cellulose, whereas A. niger Yang no. 2 could not use carboxymethyl cellulose as the sole carbon source for citric acid production. In addition, the average maximum β-glucosidase and carboxymethylcellulase productions from AT 11-2-3, AT 11-2-10, and AT 11-2-14 were about 16- and 4-folds higher than those of A. niger, respectively.

Original languageEnglish
Pages (from-to)176-186
Number of pages11
JournalApplied Biochemistry and Biotechnology
Volume143
Issue number2
DOIs
Publication statusPublished - 2007 Nov 1

Keywords

  • Aspergillus niger
  • Citric acid production
  • Intergeneric fusant
  • Polyploid
  • Trichoderma viride

ASJC Scopus subject areas

  • Biotechnology
  • Bioengineering
  • Biochemistry
  • Applied Microbiology and Biotechnology
  • Molecular Biology

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