TY - JOUR
T1 - Population profile of cytochrome P450 isozymes in the small intestine of Japanese
AU - Takenoshita-Nakaya, Sachiko
AU - Takeba, Yuko
AU - Ohta, Yuki
AU - Ootaki, Masanori
AU - Watanabe, Minoru
AU - Iiri, Taroh
AU - Otsubo, Takehito
AU - Kobayashi, Tsukasa
AU - Machida, Takafumi
AU - Watanabe, Daiki
AU - Nakamura, Yuki
AU - Yamamoto, Makoto
AU - Kumai, Toshio
AU - Kobayashi, Shinichi
AU - Matsumoto, Naoki
N1 - Publisher Copyright:
© 2018 the Japanese Society of Clinical Pharmacology and Therapeutics(JSCPT).
PY - 2018
Y1 - 2018
N2 - Cytochrome P450 (CYP) enzymes contribute to the first-pass metabolism of orally administered drugs in both the liver and human small intestine, thus influencing their bioavailability. Although the CYPs profile in the small intestine of Caucasians was reported previously, that of the Japanese population has not. We investigated CYP(1A2, 2C9, 2C19, 2D6, 3A4 and 3A5) mRNA levels and localization in the small intestine of Japanese. Localization of CYP protein secretion was observed by immunohistochemistry. CYP mRNA levels were analyzed using real-time reverse transcriptase polymerase chain reaction. In the small intestine of Japanese, CYP3A4 and CYP2C19 were the major and second most highly expressed components among these CYPs, present in 63.2% and 21.3% of our subjects, respectively. Our results showed differences between Japanese and Caucasians in the CYP profiles in the small intestine. Respective CYP isozymes in the Japanese small intestine indicate the possibility of influencing first-pass metabolism or drug interactions.
AB - Cytochrome P450 (CYP) enzymes contribute to the first-pass metabolism of orally administered drugs in both the liver and human small intestine, thus influencing their bioavailability. Although the CYPs profile in the small intestine of Caucasians was reported previously, that of the Japanese population has not. We investigated CYP(1A2, 2C9, 2C19, 2D6, 3A4 and 3A5) mRNA levels and localization in the small intestine of Japanese. Localization of CYP protein secretion was observed by immunohistochemistry. CYP mRNA levels were analyzed using real-time reverse transcriptase polymerase chain reaction. In the small intestine of Japanese, CYP3A4 and CYP2C19 were the major and second most highly expressed components among these CYPs, present in 63.2% and 21.3% of our subjects, respectively. Our results showed differences between Japanese and Caucasians in the CYP profiles in the small intestine. Respective CYP isozymes in the Japanese small intestine indicate the possibility of influencing first-pass metabolism or drug interactions.
KW - Cytochrome P450 isozyme
KW - Japanese
KW - Small intestine
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U2 - 10.3999/jscpt.49.3
DO - 10.3999/jscpt.49.3
M3 - Article
AN - SCOPUS:85042620276
SN - 0388-1601
VL - 49
SP - 3
EP - 6
JO - Japanese Journal of Clinical Pharmacology and Therapeutics
JF - Japanese Journal of Clinical Pharmacology and Therapeutics
IS - 1
ER -