Porphyrins and porphines inhibit the ribonuclease P reaction in vitro.

Yoshiaki Hori, Elena V. Bichenkova, Amanda N. Wilton, Terumichi Tanaka, Kenneth T. Douglas, Yo Kikuchi

Research output: Chapter in Book/Report/Conference proceedingChapter

3 Citations (Scopus)

Abstract

Porphyrin has been reported to bind to the T psi C stem of tRNA. This site is also recognized by ribonuclease P, which is essential and ubiquitous endoribonuclease responsible for the maturation of 5' ends of tRNA precursors. Thus, we investigated the effects of porphyrins on the in vitro reaction of ribonuclease P from Escherichia coli. The results showed that some of porphyrins inhibited the reaction more strongly than any other inhibitors reported so far. In addition to the benzimidazole inhibition that we have previously reported, these unusual substrate-binding inhibitions may provide new leads for the novel anti-bacterial reagent design.

Original languageEnglish
Title of host publicationNucleic acids research. Supplement (2001)
Pages111-112
Number of pages2
Edition2
Publication statusPublished - 2002
Externally publishedYes

Fingerprint

Ribonuclease P
Porphyrins
Endoribonucleases
RNA Precursors
Transfer RNA
Escherichia coli
In Vitro Techniques

Cite this

Hori, Y., Bichenkova, E. V., Wilton, A. N., Tanaka, T., Douglas, K. T., & Kikuchi, Y. (2002). Porphyrins and porphines inhibit the ribonuclease P reaction in vitro. In Nucleic acids research. Supplement (2001) (2 ed., pp. 111-112)

Porphyrins and porphines inhibit the ribonuclease P reaction in vitro. / Hori, Yoshiaki; Bichenkova, Elena V.; Wilton, Amanda N.; Tanaka, Terumichi; Douglas, Kenneth T.; Kikuchi, Yo.

Nucleic acids research. Supplement (2001). 2. ed. 2002. p. 111-112.

Research output: Chapter in Book/Report/Conference proceedingChapter

Hori, Y, Bichenkova, EV, Wilton, AN, Tanaka, T, Douglas, KT & Kikuchi, Y 2002, Porphyrins and porphines inhibit the ribonuclease P reaction in vitro. in Nucleic acids research. Supplement (2001). 2 edn, pp. 111-112.
Hori Y, Bichenkova EV, Wilton AN, Tanaka T, Douglas KT, Kikuchi Y. Porphyrins and porphines inhibit the ribonuclease P reaction in vitro. In Nucleic acids research. Supplement (2001). 2 ed. 2002. p. 111-112
Hori, Yoshiaki ; Bichenkova, Elena V. ; Wilton, Amanda N. ; Tanaka, Terumichi ; Douglas, Kenneth T. ; Kikuchi, Yo. / Porphyrins and porphines inhibit the ribonuclease P reaction in vitro. Nucleic acids research. Supplement (2001). 2. ed. 2002. pp. 111-112
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AU - Kikuchi, Yo

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