Positive role of the mammalian TBPIP/HOP2 protein in DMC1-mediated homologous pairing

Rima Enomoto, Takashi Kinebuchi, Makoto Sato, Hideshi Yagi, Takehiko Shibata, Hitoshi Kurumizaka, Shigeyuki Yokoyama

    Research output: Contribution to journalArticle

    30 Citations (Scopus)

    Abstract

    In meiosis, homologous recombination preferentially occurs between homologous chromosomes rather than between sister chromatids, which is opposite to the bias of mitotic recombinational repair. The TBPIP/HOP2 protein is a factor that ensures the proper pairing of homologous chromosomes during meiosis. In the present study, we found that the purified mouse TBPIP/HOP2 protein stimulated homologous pairing catalyzed by the meiotic DMC1 recombinase in vitro. In contrast, TBPIP/HOP2 did not stimulate homologous pairing by RAD51, which is another homologous pairing protein acting in both meiotic and mitotic recombination. The positive effect of TBPIP/HOP2 in the DMC1-mediated homologous pairing was only observed when TBPIP/HOP2 first binds to double-stranded DNA, not to single-stranded DNA, before the initiation of the homologous pairing reaction. Deletion analyses revealed that the C-terminal basic region of TBPIP/HOP2 is required for efficient DNA binding and is also essential for its homologous pairing stimulation activity. Therefore, these results suggest that TBPIP/HOP2 directly binds to DNA and functions as an activator for DMC1 during the homologous pairing step in meiosis.

    Original languageEnglish
    Pages (from-to)35263-35272
    Number of pages10
    JournalJournal of Biological Chemistry
    Volume279
    Issue number34
    DOIs
    Publication statusPublished - 2004 Aug 20

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    Meiosis
    Chromosomes
    DNA
    Activator Appliances
    Chromosome Pairing
    Recombinases
    Proteins
    Chromatids
    Single-Stranded DNA
    Homologous Recombination
    Genetic Recombination
    Repair

    ASJC Scopus subject areas

    • Biochemistry

    Cite this

    Enomoto, R., Kinebuchi, T., Sato, M., Yagi, H., Shibata, T., Kurumizaka, H., & Yokoyama, S. (2004). Positive role of the mammalian TBPIP/HOP2 protein in DMC1-mediated homologous pairing. Journal of Biological Chemistry, 279(34), 35263-35272. https://doi.org/10.1074/jbc.M402481200

    Positive role of the mammalian TBPIP/HOP2 protein in DMC1-mediated homologous pairing. / Enomoto, Rima; Kinebuchi, Takashi; Sato, Makoto; Yagi, Hideshi; Shibata, Takehiko; Kurumizaka, Hitoshi; Yokoyama, Shigeyuki.

    In: Journal of Biological Chemistry, Vol. 279, No. 34, 20.08.2004, p. 35263-35272.

    Research output: Contribution to journalArticle

    Enomoto, R, Kinebuchi, T, Sato, M, Yagi, H, Shibata, T, Kurumizaka, H & Yokoyama, S 2004, 'Positive role of the mammalian TBPIP/HOP2 protein in DMC1-mediated homologous pairing', Journal of Biological Chemistry, vol. 279, no. 34, pp. 35263-35272. https://doi.org/10.1074/jbc.M402481200
    Enomoto R, Kinebuchi T, Sato M, Yagi H, Shibata T, Kurumizaka H et al. Positive role of the mammalian TBPIP/HOP2 protein in DMC1-mediated homologous pairing. Journal of Biological Chemistry. 2004 Aug 20;279(34):35263-35272. https://doi.org/10.1074/jbc.M402481200
    Enomoto, Rima ; Kinebuchi, Takashi ; Sato, Makoto ; Yagi, Hideshi ; Shibata, Takehiko ; Kurumizaka, Hitoshi ; Yokoyama, Shigeyuki. / Positive role of the mammalian TBPIP/HOP2 protein in DMC1-mediated homologous pairing. In: Journal of Biological Chemistry. 2004 ; Vol. 279, No. 34. pp. 35263-35272.
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