Positron-labeled antioxidant 6-deoxy-6-[18F]fluoro-L-ascorbic acid: Increased uptake in transient global ischemic rat brain

Fumihiko Yamamoto, Shigenobu Shibata, Shigenori Watanabe, Kouji Masuda, Minoru Maeda

Research output: Contribution to journalArticle

10 Citations (Scopus)

Abstract

The in vivo uptake and distribution of 6-deoxy-6-[18F]fluoro-L-ascorbic acid (18F-DFA) were investigated in rat brains following postischemic reperfusion. Global cerebral ischemia was induced in male Wistar rats for 20 min by occlusion of four major arteries. Two time paints were chosen for 18F-DFA injection to rats subjected to cerebral ischemia, at the start of recirculation and 5 days following recirculation. The rats were then killed at 2 h after tail-vein administration of 18F-DFA and tissue radioactivity concentration was determined. Increased uptake of radioactivity in particular brain regions, including the cerebral cortex, hypothalamus, and amygdala following injection of 18F-DFA, compared to the sham operated control, was observed 5 days after reperfusion. Similar results were also obtained in in vitro experiments using brain slices. Abnormal in vivo accumulation of 45Ca, a marker of regional postischemic injury, was observed in these brain regions in tissue dissection experiments. Furthermore, metabolite analysis of nonradioactive DFA using 19F-NMR showed that DFA remained intact in the postischemic reperfusion brain. The present results indicate that 18F-DFA increasingly accumulates in damaged regions of postischemic reperfusion brain.

Original languageEnglish
Pages (from-to)479-486
Number of pages8
JournalNuclear Medicine and Biology
Volume23
Issue number4
DOIs
Publication statusPublished - 1996 May

Keywords

  • 6-Deoxy-6-fluoro-L-ascorbic acid
  • Brain
  • F-NMR
  • Fluorine-18
  • In vivo
  • Ischemia

ASJC Scopus subject areas

  • Molecular Medicine
  • Radiology Nuclear Medicine and imaging
  • Cancer Research

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