Possible hormonal interaction for eliciting courtship behavior in the male newt, Cynops pyrrhogaster

Fumiyo Toyoda, Itaru Hasunuma, Tomoaki Nakada, Shogo Haraguchi, Kazuyoshi Tsutsui, Sakae Kikuyama

    Research output: Contribution to journalArticle

    3 Citations (Scopus)

    Abstract

    Reproductive behavior in amphibians, as in other vertebrate animals, is under the control of multiple hormonal substances. Prolactin (PRL), arginine vasotocin (AVT), androgen, and 7α-hydroxypregnenolone (7α-OH PREG), four such substances with hormonal activity, are known to be involved in the expression of the tail vibration behavior which is the initial step of courtship performed by the male newt, Cynops pyrrhogaster. As current information on the interaction(s) between these hormones in terms of eliciting tail vibration behavior is limited, we have investigated whether the decline of expression of tail vibration behavior due to suppression of the activity of any one of these hormones can be restored by supplying any one of the other three hormones exogenously. Expression of the behavior was determined in terms of incidence (% of test animals exhibiting the behavior) and frequency (number of times that the behavior was repeated during the test period). Neither PRL nor androgen restored the decline in the incidence and frequency of the tail vibration behavior caused by the suppression of the activity of any one of other three hormones. AVT completely restored both the anti-PRL antibody-induced and flutamide (an androgen receptor antagonist)-induced, but not ketoconazole (an inhibitor of the steroidogenic CYP enzymes)-induced decline in the incidence and frequency of the tail vibration behavior. The neurosteroid, 7α-OH PREG, failed to restore flutamide-induced decline in the incidence and frequency of the behavior. However, it was able to restore both anti-PRL antibody-induced and AVT receptor antagonist-induced decline in the incidence, but not in the frequency of the behavior. In another experiment designed to see the activity of hormones enhancing the frequency of the tail vibration behavior, AVT was revealed to be more potent than 7α-OH PREG. The role of each hormonal substance in determining the expression of the tail vibration behavior was discussed based on the results.

    Original languageEnglish
    Pages (from-to)96-103
    Number of pages8
    JournalGeneral and Comparative Endocrinology
    Volume224
    DOIs
    Publication statusPublished - 2015 Dec 1

    Fingerprint

    Salamandridae
    Courtship
    salamanders and newts
    vibration
    courtship
    arginine vasotocin
    tail
    Vibration
    prolactin
    hormones
    Tail
    incidence
    Vasotocin
    Prolactin
    Hormones
    androgens
    Incidence
    Flutamide
    antagonists
    ketoconazole

    Keywords

    • Courtship behavior
    • Hormones
    • Newt

    ASJC Scopus subject areas

    • Endocrinology

    Cite this

    Possible hormonal interaction for eliciting courtship behavior in the male newt, Cynops pyrrhogaster. / Toyoda, Fumiyo; Hasunuma, Itaru; Nakada, Tomoaki; Haraguchi, Shogo; Tsutsui, Kazuyoshi; Kikuyama, Sakae.

    In: General and Comparative Endocrinology, Vol. 224, 01.12.2015, p. 96-103.

    Research output: Contribution to journalArticle

    Toyoda, Fumiyo ; Hasunuma, Itaru ; Nakada, Tomoaki ; Haraguchi, Shogo ; Tsutsui, Kazuyoshi ; Kikuyama, Sakae. / Possible hormonal interaction for eliciting courtship behavior in the male newt, Cynops pyrrhogaster. In: General and Comparative Endocrinology. 2015 ; Vol. 224. pp. 96-103.
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