Possible involvement of Escherichia coli 23S ribosomal RNA in peptide bond formation

Itaru Nitta, Takuya Ueda, Kimitsuna Watanabe

Research output: Contribution to journalArticle

28 Citations (Scopus)

Abstract

Experimental results are presented suggesting that 23S rRNA is directly involved in the peptide bond formation usually performed on the ribosome. Although several reports have indicated that the eubacterial peptidyltransferase reaction does not necessarily require all the ribosomal proteins, the reconstitution of peptidyltransferase activity by a naked 23S rRNA without the help of any of the ribosomal proteins has not been reported previously. It is demonstrated that an E. coli 23S rRNA transcript synthesized by T7 RNA polymerase in vitro was able to promote peptide bond formation in the presence of 0.5% SDS. The reaction was inhibited by the peptidyltransferase-specific antibiotics chloramphenicol and carbomycin, and by digestion with RNases A and T1. Site-directed mutageneses at two highly conserved regions close to the peptidyltransferase center ring, G2252 to U2252 and C2507G2581 to U2507A2581, also suppressed peptide bond formation. These findings strongly suggest that 23S rRNA is the peptidyltransferase itself.

Original languageEnglish
Pages (from-to)257-267
Number of pages11
JournalRNA
Volume4
Issue number3
Publication statusPublished - 1998 Mar 1
Externally publishedYes

Fingerprint

23S Ribosomal RNA
Peptidyl Transferases
Escherichia coli
Peptides
Ribosomal Proteins
Ribonuclease T1
Pancreatic Ribonuclease
Chloramphenicol
Site-Directed Mutagenesis
Ribosomes
Digestion
Anti-Bacterial Agents

Keywords

  • Carbomycin
  • Chloramphenicol
  • N-acetylphenylalanyl- tRNA(Phe)
  • Peptidyltransferase
  • Phenylalanyl-tRNA(Phe)
  • Site-directed mutation

ASJC Scopus subject areas

  • Molecular Biology

Cite this

Possible involvement of Escherichia coli 23S ribosomal RNA in peptide bond formation. / Nitta, Itaru; Ueda, Takuya; Watanabe, Kimitsuna.

In: RNA, Vol. 4, No. 3, 01.03.1998, p. 257-267.

Research output: Contribution to journalArticle

Nitta, Itaru ; Ueda, Takuya ; Watanabe, Kimitsuna. / Possible involvement of Escherichia coli 23S ribosomal RNA in peptide bond formation. In: RNA. 1998 ; Vol. 4, No. 3. pp. 257-267.
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