Possible role of GnIH as a mediator between adiposity and impaired testicular function

Shabana Anjum, Amitabh Krishna, Kazuyoshi Tsutsui

    Research output: Contribution to journalArticle

    11 Citations (Scopus)

    Abstract

    The aim of the present study was to evaluate the roles of gonadotropin-inhibitory hormone (GnIH) as an endocrine link between increasing adiposity and impaired testicular function in mice. To achieve this, the effect of GnIH on changes in nutrients uptake and hormonal synthesis/action in the adipose tissue and testis was investigated simultaneously by in vivo study and separately by in vitro study. Mice were treated in vivo with different doses of GnIH for 8 days. In the in vitro study, adipose tissue and testes of mice were cultured with different doses of GnIH with or without insulin or LH for 24 h at 37°C. The GnIH treatment in vivo showed increased food intake, upregulation of glucose transporter 4 (GLUT4), and increased uptake of triglycerides (TGs) in the adipose tissue. These changes may be responsible for increased accumulation of fat in white adipose tissue, resulting in increase in the body mass. Contrary to the adipose tissue, treatment with GnIH both in vivo and in vitro showed decreased uptake of glucose by downregulation of glucose transporter 8 (GLUT8) expressions in the testis, which in turn resulted in the decreased synthesis of testosterone. The GnIH treatment in vivo also showed the decreased expression of insulin receptor protein in the testis, which may also be responsible for the decreased testicular activity in the mice. These findings thus suggest that GnIH increases the uptake of glucose and TGs in the adipose tissue, resulting in increased accumulation of fat, whereas simultaneously in the testis, GnIH suppressed the GLUT8-mediated glucose uptake, which in turn may be responsible for decreased testosterone synthesis. This study thus demonstrates GnIH as mediator of increasing adiposity and impaired testicular function in mice.

    Original languageEnglish
    Article number6
    JournalFrontiers in Endocrinology
    Volume7
    Issue numberFEB
    DOIs
    Publication statusPublished - 2016

    Fingerprint

    Adiposity
    Gonadotropins
    Hormones
    Adipose Tissue
    Testis
    Facilitative Glucose Transport Proteins
    Glucose
    Testosterone
    Triglycerides
    Fats
    White Adipose Tissue
    Insulin Receptor
    Up-Regulation
    Down-Regulation
    Eating
    Insulin

    Keywords

    • Adipose tissue
    • GLUT4
    • GLUT8
    • GnIH
    • Testis

    ASJC Scopus subject areas

    • Endocrinology, Diabetes and Metabolism

    Cite this

    Possible role of GnIH as a mediator between adiposity and impaired testicular function. / Anjum, Shabana; Krishna, Amitabh; Tsutsui, Kazuyoshi.

    In: Frontiers in Endocrinology, Vol. 7, No. FEB, 6, 2016.

    Research output: Contribution to journalArticle

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