Post-exercise ingestion of different amounts of protein affects plasma insulin concentration in humans

Masashi Morifuji, Tomoko Aoyama, Ai Nakata, Chiaki Sambongi, Jinichiro Koga, Kunihiko Kurihara, Minoru Kanegae, Katsuhiko Suzuki, Mitsuru Higuchi

Research output: Contribution to journalArticle

5 Citations (Scopus)

Abstract

The synergistic stimulating effect of combined intake of carbohydrate and protein on plasma insulin concentration has been reported previously. However, it remains unclear whether the amount of protein ingested after exercise affects the concentrations of plasma insulin and amino acids. This study of trained men compared the effects of post-exercise co-ingestion of carbohydrate plus different amounts of whey protein hydrolysates (WPHs) with carbohydrate alone on (1) blood biochemical parameters of carbohydrate metabolism during the post-exercise phase, and (2) endurance performance. Eight trained men exercised continuously for 70 min. Immediately after exercise and 30, 60, 90, and 120 min later, the participants received supplements containing: (1) 17.5 g carbohydrate, (2) 3.0 g WPHs and 17.5 g carbohydrate (L-WPH), or (3) 8.0 g WPHs and 17.5 g carbohydrate (H-WPH). After a 2-h recovery period, the participants performed an endurance performance test. The concentrations of blood glucose were lower and plasma insulin significantly higher in the H-WPH trial compared with the carbohydrate trial. The concentrations of plasma amino acids were increased in a dose-dependent manner following ingestion of different amounts of WPHs with carbohydrate. Endurance performance was not significantly different between the three trials. Co-ingestion of carbohydrate and H-WPH was more effective than ingestion of carbohydrate alone for stimulating insulin secretion and increasing the availability of plasma amino acids. These results suggest that plasma concentrations of amino acids during the recovery period are determined by the amount of dietary protein ingested, and that it is necessary to increase the concentration of plasma amino acids above a certain level to stimulate insulin secretion.

Original languageEnglish
Pages (from-to)152-160
Number of pages9
JournalEuropean Journal of Sport Science
Volume12
Issue number2
DOIs
Publication statusPublished - 2012 Mar

Fingerprint

Protein Hydrolysates
Blood Proteins
Eating
Carbohydrates
Exercise
Insulin
Amino Acids
Dietary Proteins
Whey Proteins
Carbohydrate Metabolism
Blood Glucose

Keywords

  • blood glucose
  • plasma amino acids
  • plasma insulin
  • post-exercise
  • Protein dose

ASJC Scopus subject areas

  • Physical Therapy, Sports Therapy and Rehabilitation
  • Orthopedics and Sports Medicine

Cite this

Morifuji, M., Aoyama, T., Nakata, A., Sambongi, C., Koga, J., Kurihara, K., ... Higuchi, M. (2012). Post-exercise ingestion of different amounts of protein affects plasma insulin concentration in humans. European Journal of Sport Science, 12(2), 152-160. https://doi.org/10.1080/17461391.2010.551415

Post-exercise ingestion of different amounts of protein affects plasma insulin concentration in humans. / Morifuji, Masashi; Aoyama, Tomoko; Nakata, Ai; Sambongi, Chiaki; Koga, Jinichiro; Kurihara, Kunihiko; Kanegae, Minoru; Suzuki, Katsuhiko; Higuchi, Mitsuru.

In: European Journal of Sport Science, Vol. 12, No. 2, 03.2012, p. 152-160.

Research output: Contribution to journalArticle

Morifuji, M, Aoyama, T, Nakata, A, Sambongi, C, Koga, J, Kurihara, K, Kanegae, M, Suzuki, K & Higuchi, M 2012, 'Post-exercise ingestion of different amounts of protein affects plasma insulin concentration in humans', European Journal of Sport Science, vol. 12, no. 2, pp. 152-160. https://doi.org/10.1080/17461391.2010.551415
Morifuji, Masashi ; Aoyama, Tomoko ; Nakata, Ai ; Sambongi, Chiaki ; Koga, Jinichiro ; Kurihara, Kunihiko ; Kanegae, Minoru ; Suzuki, Katsuhiko ; Higuchi, Mitsuru. / Post-exercise ingestion of different amounts of protein affects plasma insulin concentration in humans. In: European Journal of Sport Science. 2012 ; Vol. 12, No. 2. pp. 152-160.
@article{b6588569f2a84f93a36ddc009094d629,
title = "Post-exercise ingestion of different amounts of protein affects plasma insulin concentration in humans",
abstract = "The synergistic stimulating effect of combined intake of carbohydrate and protein on plasma insulin concentration has been reported previously. However, it remains unclear whether the amount of protein ingested after exercise affects the concentrations of plasma insulin and amino acids. This study of trained men compared the effects of post-exercise co-ingestion of carbohydrate plus different amounts of whey protein hydrolysates (WPHs) with carbohydrate alone on (1) blood biochemical parameters of carbohydrate metabolism during the post-exercise phase, and (2) endurance performance. Eight trained men exercised continuously for 70 min. Immediately after exercise and 30, 60, 90, and 120 min later, the participants received supplements containing: (1) 17.5 g carbohydrate, (2) 3.0 g WPHs and 17.5 g carbohydrate (L-WPH), or (3) 8.0 g WPHs and 17.5 g carbohydrate (H-WPH). After a 2-h recovery period, the participants performed an endurance performance test. The concentrations of blood glucose were lower and plasma insulin significantly higher in the H-WPH trial compared with the carbohydrate trial. The concentrations of plasma amino acids were increased in a dose-dependent manner following ingestion of different amounts of WPHs with carbohydrate. Endurance performance was not significantly different between the three trials. Co-ingestion of carbohydrate and H-WPH was more effective than ingestion of carbohydrate alone for stimulating insulin secretion and increasing the availability of plasma amino acids. These results suggest that plasma concentrations of amino acids during the recovery period are determined by the amount of dietary protein ingested, and that it is necessary to increase the concentration of plasma amino acids above a certain level to stimulate insulin secretion.",
keywords = "blood glucose, plasma amino acids, plasma insulin, post-exercise, Protein dose",
author = "Masashi Morifuji and Tomoko Aoyama and Ai Nakata and Chiaki Sambongi and Jinichiro Koga and Kunihiko Kurihara and Minoru Kanegae and Katsuhiko Suzuki and Mitsuru Higuchi",
year = "2012",
month = "3",
doi = "10.1080/17461391.2010.551415",
language = "English",
volume = "12",
pages = "152--160",
journal = "European Journal of Sport Science",
issn = "1746-1391",
publisher = "Taylor and Francis Ltd.",
number = "2",

}

TY - JOUR

T1 - Post-exercise ingestion of different amounts of protein affects plasma insulin concentration in humans

AU - Morifuji, Masashi

AU - Aoyama, Tomoko

AU - Nakata, Ai

AU - Sambongi, Chiaki

AU - Koga, Jinichiro

AU - Kurihara, Kunihiko

AU - Kanegae, Minoru

AU - Suzuki, Katsuhiko

AU - Higuchi, Mitsuru

PY - 2012/3

Y1 - 2012/3

N2 - The synergistic stimulating effect of combined intake of carbohydrate and protein on plasma insulin concentration has been reported previously. However, it remains unclear whether the amount of protein ingested after exercise affects the concentrations of plasma insulin and amino acids. This study of trained men compared the effects of post-exercise co-ingestion of carbohydrate plus different amounts of whey protein hydrolysates (WPHs) with carbohydrate alone on (1) blood biochemical parameters of carbohydrate metabolism during the post-exercise phase, and (2) endurance performance. Eight trained men exercised continuously for 70 min. Immediately after exercise and 30, 60, 90, and 120 min later, the participants received supplements containing: (1) 17.5 g carbohydrate, (2) 3.0 g WPHs and 17.5 g carbohydrate (L-WPH), or (3) 8.0 g WPHs and 17.5 g carbohydrate (H-WPH). After a 2-h recovery period, the participants performed an endurance performance test. The concentrations of blood glucose were lower and plasma insulin significantly higher in the H-WPH trial compared with the carbohydrate trial. The concentrations of plasma amino acids were increased in a dose-dependent manner following ingestion of different amounts of WPHs with carbohydrate. Endurance performance was not significantly different between the three trials. Co-ingestion of carbohydrate and H-WPH was more effective than ingestion of carbohydrate alone for stimulating insulin secretion and increasing the availability of plasma amino acids. These results suggest that plasma concentrations of amino acids during the recovery period are determined by the amount of dietary protein ingested, and that it is necessary to increase the concentration of plasma amino acids above a certain level to stimulate insulin secretion.

AB - The synergistic stimulating effect of combined intake of carbohydrate and protein on plasma insulin concentration has been reported previously. However, it remains unclear whether the amount of protein ingested after exercise affects the concentrations of plasma insulin and amino acids. This study of trained men compared the effects of post-exercise co-ingestion of carbohydrate plus different amounts of whey protein hydrolysates (WPHs) with carbohydrate alone on (1) blood biochemical parameters of carbohydrate metabolism during the post-exercise phase, and (2) endurance performance. Eight trained men exercised continuously for 70 min. Immediately after exercise and 30, 60, 90, and 120 min later, the participants received supplements containing: (1) 17.5 g carbohydrate, (2) 3.0 g WPHs and 17.5 g carbohydrate (L-WPH), or (3) 8.0 g WPHs and 17.5 g carbohydrate (H-WPH). After a 2-h recovery period, the participants performed an endurance performance test. The concentrations of blood glucose were lower and plasma insulin significantly higher in the H-WPH trial compared with the carbohydrate trial. The concentrations of plasma amino acids were increased in a dose-dependent manner following ingestion of different amounts of WPHs with carbohydrate. Endurance performance was not significantly different between the three trials. Co-ingestion of carbohydrate and H-WPH was more effective than ingestion of carbohydrate alone for stimulating insulin secretion and increasing the availability of plasma amino acids. These results suggest that plasma concentrations of amino acids during the recovery period are determined by the amount of dietary protein ingested, and that it is necessary to increase the concentration of plasma amino acids above a certain level to stimulate insulin secretion.

KW - blood glucose

KW - plasma amino acids

KW - plasma insulin

KW - post-exercise

KW - Protein dose

UR - http://www.scopus.com/inward/record.url?scp=84863372501&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=84863372501&partnerID=8YFLogxK

U2 - 10.1080/17461391.2010.551415

DO - 10.1080/17461391.2010.551415

M3 - Article

VL - 12

SP - 152

EP - 160

JO - European Journal of Sport Science

JF - European Journal of Sport Science

SN - 1746-1391

IS - 2

ER -