Postnatal apoptosis, development, and sex difference in the lateral septum of rats

Shinji Tsukahara, Kouichi Inami, Fumihiko Maekawa, Masaki Kakeyama, Toshifumi Yokoyama, Midori Yuji, Hiroshi Kitagawa, Yasuyuki Kannan, Korehito Yamanouchi

Research output: Contribution to journalArticle

15 Citations (Scopus)

Abstract

To determine whether apoptosis is involved in the formation of the structure and morphological sex difference of the lateral septum (LS), the postnatal developmental changes in the number of apoptotic cells were examined in the LS on postnatal day 1 (PD1 = birth day), 4, 6, 8, 11, 16, and 31 in male and female rats. Apoptotic cells were immunohistochemically detected by antibody against single-stranded DNA (ssDNA) or active caspase-3. The volume of the LS was also measured and was found to increase with age. The number of apoptotic cells detected by anti-ssDNA in the LS increased from PD1 to PD8 but decreased after PD11. Also, the LS was divided into dorsal, intermediate, and ventral parts (LSd, LSi, and LSv), and the volume and number of ssDNA-immunoreactive cells in each part were measured on PD6, 8, 11, 16, and 31. In both sexes, a large number of ssDNA-immunoreactive cells was found in the LSd and LSi on PD8 (but not on PD6) and in the LSv on PD6 and PD8. On PD6, the number of active caspase-3-immunoreactive cells was significantly greater in the LSv than in the LSd or LSi, in both sexes. Only the LSi of males had a high number of ssDNA-immunoreacitve cells on PD16; the number was significantly greater than that of females of the same age. However, there was no significant sex difference in the number of active caspase-3-immunoreacitve cells in the LSi on PD16. On PD31, the volume of the LSi was significantly greater in females than in males. There was no sex difference in volume or number of apoptotic cells in the LSd or LSv. These findings indicate that loss of cells due to apoptosis, which is partially caused by activation of caspase-3, occurs in the LS during postnatal development, with regional differences. They also indicate that sex difference in caspase-3-independent apoptosis contributes to morphological sexual differentiation of the LSi.

Original languageEnglish
Pages (from-to)177-187
Number of pages11
JournalJournal of Comparative Neurology
Volume475
Issue number2
DOIs
Publication statusPublished - 2004 Jul 19
Externally publishedYes

Fingerprint

Sex Characteristics
Single-Stranded DNA
Apoptosis
Caspase 3
Cell Count
Sex Differentiation
Parturition
Antibodies

Keywords

  • Active caspase-3
  • Sexual differentiation
  • Sexual dimorphism
  • Single-stranded DNA

ASJC Scopus subject areas

  • Neuroscience(all)

Cite this

Tsukahara, S., Inami, K., Maekawa, F., Kakeyama, M., Yokoyama, T., Yuji, M., ... Yamanouchi, K. (2004). Postnatal apoptosis, development, and sex difference in the lateral septum of rats. Journal of Comparative Neurology, 475(2), 177-187. https://doi.org/10.1002/cne.20184

Postnatal apoptosis, development, and sex difference in the lateral septum of rats. / Tsukahara, Shinji; Inami, Kouichi; Maekawa, Fumihiko; Kakeyama, Masaki; Yokoyama, Toshifumi; Yuji, Midori; Kitagawa, Hiroshi; Kannan, Yasuyuki; Yamanouchi, Korehito.

In: Journal of Comparative Neurology, Vol. 475, No. 2, 19.07.2004, p. 177-187.

Research output: Contribution to journalArticle

Tsukahara, S, Inami, K, Maekawa, F, Kakeyama, M, Yokoyama, T, Yuji, M, Kitagawa, H, Kannan, Y & Yamanouchi, K 2004, 'Postnatal apoptosis, development, and sex difference in the lateral septum of rats', Journal of Comparative Neurology, vol. 475, no. 2, pp. 177-187. https://doi.org/10.1002/cne.20184
Tsukahara, Shinji ; Inami, Kouichi ; Maekawa, Fumihiko ; Kakeyama, Masaki ; Yokoyama, Toshifumi ; Yuji, Midori ; Kitagawa, Hiroshi ; Kannan, Yasuyuki ; Yamanouchi, Korehito. / Postnatal apoptosis, development, and sex difference in the lateral septum of rats. In: Journal of Comparative Neurology. 2004 ; Vol. 475, No. 2. pp. 177-187.
@article{e62e84ed2a01443281c238fd7cfa932c,
title = "Postnatal apoptosis, development, and sex difference in the lateral septum of rats",
abstract = "To determine whether apoptosis is involved in the formation of the structure and morphological sex difference of the lateral septum (LS), the postnatal developmental changes in the number of apoptotic cells were examined in the LS on postnatal day 1 (PD1 = birth day), 4, 6, 8, 11, 16, and 31 in male and female rats. Apoptotic cells were immunohistochemically detected by antibody against single-stranded DNA (ssDNA) or active caspase-3. The volume of the LS was also measured and was found to increase with age. The number of apoptotic cells detected by anti-ssDNA in the LS increased from PD1 to PD8 but decreased after PD11. Also, the LS was divided into dorsal, intermediate, and ventral parts (LSd, LSi, and LSv), and the volume and number of ssDNA-immunoreactive cells in each part were measured on PD6, 8, 11, 16, and 31. In both sexes, a large number of ssDNA-immunoreactive cells was found in the LSd and LSi on PD8 (but not on PD6) and in the LSv on PD6 and PD8. On PD6, the number of active caspase-3-immunoreactive cells was significantly greater in the LSv than in the LSd or LSi, in both sexes. Only the LSi of males had a high number of ssDNA-immunoreacitve cells on PD16; the number was significantly greater than that of females of the same age. However, there was no significant sex difference in the number of active caspase-3-immunoreacitve cells in the LSi on PD16. On PD31, the volume of the LSi was significantly greater in females than in males. There was no sex difference in volume or number of apoptotic cells in the LSd or LSv. These findings indicate that loss of cells due to apoptosis, which is partially caused by activation of caspase-3, occurs in the LS during postnatal development, with regional differences. They also indicate that sex difference in caspase-3-independent apoptosis contributes to morphological sexual differentiation of the LSi.",
keywords = "Active caspase-3, Sexual differentiation, Sexual dimorphism, Single-stranded DNA",
author = "Shinji Tsukahara and Kouichi Inami and Fumihiko Maekawa and Masaki Kakeyama and Toshifumi Yokoyama and Midori Yuji and Hiroshi Kitagawa and Yasuyuki Kannan and Korehito Yamanouchi",
year = "2004",
month = "7",
day = "19",
doi = "10.1002/cne.20184",
language = "English",
volume = "475",
pages = "177--187",
journal = "Journal of Comparative Neurology",
issn = "0021-9967",
publisher = "Wiley-Liss Inc.",
number = "2",

}

TY - JOUR

T1 - Postnatal apoptosis, development, and sex difference in the lateral septum of rats

AU - Tsukahara, Shinji

AU - Inami, Kouichi

AU - Maekawa, Fumihiko

AU - Kakeyama, Masaki

AU - Yokoyama, Toshifumi

AU - Yuji, Midori

AU - Kitagawa, Hiroshi

AU - Kannan, Yasuyuki

AU - Yamanouchi, Korehito

PY - 2004/7/19

Y1 - 2004/7/19

N2 - To determine whether apoptosis is involved in the formation of the structure and morphological sex difference of the lateral septum (LS), the postnatal developmental changes in the number of apoptotic cells were examined in the LS on postnatal day 1 (PD1 = birth day), 4, 6, 8, 11, 16, and 31 in male and female rats. Apoptotic cells were immunohistochemically detected by antibody against single-stranded DNA (ssDNA) or active caspase-3. The volume of the LS was also measured and was found to increase with age. The number of apoptotic cells detected by anti-ssDNA in the LS increased from PD1 to PD8 but decreased after PD11. Also, the LS was divided into dorsal, intermediate, and ventral parts (LSd, LSi, and LSv), and the volume and number of ssDNA-immunoreactive cells in each part were measured on PD6, 8, 11, 16, and 31. In both sexes, a large number of ssDNA-immunoreactive cells was found in the LSd and LSi on PD8 (but not on PD6) and in the LSv on PD6 and PD8. On PD6, the number of active caspase-3-immunoreactive cells was significantly greater in the LSv than in the LSd or LSi, in both sexes. Only the LSi of males had a high number of ssDNA-immunoreacitve cells on PD16; the number was significantly greater than that of females of the same age. However, there was no significant sex difference in the number of active caspase-3-immunoreacitve cells in the LSi on PD16. On PD31, the volume of the LSi was significantly greater in females than in males. There was no sex difference in volume or number of apoptotic cells in the LSd or LSv. These findings indicate that loss of cells due to apoptosis, which is partially caused by activation of caspase-3, occurs in the LS during postnatal development, with regional differences. They also indicate that sex difference in caspase-3-independent apoptosis contributes to morphological sexual differentiation of the LSi.

AB - To determine whether apoptosis is involved in the formation of the structure and morphological sex difference of the lateral septum (LS), the postnatal developmental changes in the number of apoptotic cells were examined in the LS on postnatal day 1 (PD1 = birth day), 4, 6, 8, 11, 16, and 31 in male and female rats. Apoptotic cells were immunohistochemically detected by antibody against single-stranded DNA (ssDNA) or active caspase-3. The volume of the LS was also measured and was found to increase with age. The number of apoptotic cells detected by anti-ssDNA in the LS increased from PD1 to PD8 but decreased after PD11. Also, the LS was divided into dorsal, intermediate, and ventral parts (LSd, LSi, and LSv), and the volume and number of ssDNA-immunoreactive cells in each part were measured on PD6, 8, 11, 16, and 31. In both sexes, a large number of ssDNA-immunoreactive cells was found in the LSd and LSi on PD8 (but not on PD6) and in the LSv on PD6 and PD8. On PD6, the number of active caspase-3-immunoreactive cells was significantly greater in the LSv than in the LSd or LSi, in both sexes. Only the LSi of males had a high number of ssDNA-immunoreacitve cells on PD16; the number was significantly greater than that of females of the same age. However, there was no significant sex difference in the number of active caspase-3-immunoreacitve cells in the LSi on PD16. On PD31, the volume of the LSi was significantly greater in females than in males. There was no sex difference in volume or number of apoptotic cells in the LSd or LSv. These findings indicate that loss of cells due to apoptosis, which is partially caused by activation of caspase-3, occurs in the LS during postnatal development, with regional differences. They also indicate that sex difference in caspase-3-independent apoptosis contributes to morphological sexual differentiation of the LSi.

KW - Active caspase-3

KW - Sexual differentiation

KW - Sexual dimorphism

KW - Single-stranded DNA

UR - http://www.scopus.com/inward/record.url?scp=3042847166&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=3042847166&partnerID=8YFLogxK

U2 - 10.1002/cne.20184

DO - 10.1002/cne.20184

M3 - Article

C2 - 15211459

AN - SCOPUS:3042847166

VL - 475

SP - 177

EP - 187

JO - Journal of Comparative Neurology

JF - Journal of Comparative Neurology

SN - 0021-9967

IS - 2

ER -