Potassium-regulated immobilization of cortisol aptamer for field-effect transistor biosensor to detect changes in charge distribution with aptamer transformation

Shigeki Kuroiwa, Hiroki Hayashi, Ryo Toyama, Naoto Kaneko, Katsunori Horii, Keishi Ohashi, Toshiyuki Momma, Tetsuya Osaka

Research output: Contribution to journalArticlepeer-review

Abstract

Salivary cortisol concentration and its circadian variation were detected by optimizing the ionic concentration of a solution during the immobilization of aptamers on a field-effect transistor biosensor. This was achieved by modifying our previously developed technique of detecting uncharged cortisol using the transformation of negatively charged aptamers by controlling the aptamer spacing. This spacing control was achieved by synthesizing pseudo-cortisol-binding aptamers with a guaninequadruplex during the immobilization of the aptamers in a highly concentrated solution of K+.

Original languageEnglish
Pages (from-to)892-895
Number of pages4
JournalChemistry Letters
Volume50
Issue number5
DOIs
Publication statusPublished - 2021 May

Keywords

  • Cortisol aptamer
  • Field-effect transistor
  • Guanine-quadruplex

ASJC Scopus subject areas

  • Chemistry(all)

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