Potent synchronization of peripheral circadian clocks by glucocorticoid injections in PER2::LUC-Clock/Clock mice

Mayo Kamagata, Yuko Ikeda, Hiroyuki Sasaki, Yuta Hattori, Shinnosuke Yasuda, Shiho Iwami, Miku Tsubosaka, Ryosuke Ishikawa, Ai Todoh, Konomi Tamura, Yu Tahara, Shigenobu Shibata

Research output: Contribution to journalArticle

4 Citations (Scopus)

Abstract

In mammals, the central clock (the suprachiasmatic nuclei, SCN) is entrained mainly by the light-dark cycle, whereas peripheral clocks in the peripheral tissues are entrained/synchronized by multiple factors, including feeding patterns and endocrine hormones such as glucocorticoids. Clock-mutant mice (Clock/Clock), which have a mutation in a core clock gene, show potent phase resetting in response to light pulses compared with wild-type (WT) mice, owing to the damped and flexible oscillator in the SCN. However, the phase resetting of the peripheral clocks in Clock/Clock mice has not been elucidated. Here, we characterized the peripheral clock gene synchronization in Clock/Clock mice by daily injections of a synthetic glucocorticoid (dexamethasone, DEX) by monitoring in vivo PER2::LUCIFERASE bioluminescence. Compared with WT mice, the Clock/Clock mice showed significantly decreased bioluminescence and peripheral clock rhythms with decreased amplitudes and delayed phases. In addition, the DEX injections increased the amplitudes and advanced the phases. In order to examine the robustness of the internal oscillator, T-cycle experiments involving DEX stimulations with 24- or 30-h intervals were performed. The Clock/Clock mice synchronized to the 30-h T-cycle stimulation, which suggested that the peripheral clocks in the Clock/Clock mice had increased synchronizing ability upon DEX stimulation, to that of circadian and hour-glass type oscillations, because of weak internal clock oscillators.

Original languageEnglish
Pages (from-to)1067-1082
Number of pages16
JournalChronobiology International
Volume34
Issue number8
DOIs
Publication statusPublished - 2017 Sep 14

Fingerprint

Circadian Clocks
Glucocorticoids
Injections
Dexamethasone
Suprachiasmatic Nucleus
Aptitude
Photoperiod
Feeding Behavior
Genes
Glass
Mammals
Hormones
Light
Mutation

Keywords

  • Circadian rhythm
  • Clock mutation
  • glucocorticoid
  • synchronizing ability

ASJC Scopus subject areas

  • Physiology
  • Physiology (medical)

Cite this

Potent synchronization of peripheral circadian clocks by glucocorticoid injections in PER2::LUC-Clock/Clock mice. / Kamagata, Mayo; Ikeda, Yuko; Sasaki, Hiroyuki; Hattori, Yuta; Yasuda, Shinnosuke; Iwami, Shiho; Tsubosaka, Miku; Ishikawa, Ryosuke; Todoh, Ai; Tamura, Konomi; Tahara, Yu; Shibata, Shigenobu.

In: Chronobiology International, Vol. 34, No. 8, 14.09.2017, p. 1067-1082.

Research output: Contribution to journalArticle

Kamagata, M, Ikeda, Y, Sasaki, H, Hattori, Y, Yasuda, S, Iwami, S, Tsubosaka, M, Ishikawa, R, Todoh, A, Tamura, K, Tahara, Y & Shibata, S 2017, 'Potent synchronization of peripheral circadian clocks by glucocorticoid injections in PER2::LUC-Clock/Clock mice', Chronobiology International, vol. 34, no. 8, pp. 1067-1082. https://doi.org/10.1080/07420528.2017.1338716
Kamagata, Mayo ; Ikeda, Yuko ; Sasaki, Hiroyuki ; Hattori, Yuta ; Yasuda, Shinnosuke ; Iwami, Shiho ; Tsubosaka, Miku ; Ishikawa, Ryosuke ; Todoh, Ai ; Tamura, Konomi ; Tahara, Yu ; Shibata, Shigenobu. / Potent synchronization of peripheral circadian clocks by glucocorticoid injections in PER2::LUC-Clock/Clock mice. In: Chronobiology International. 2017 ; Vol. 34, No. 8. pp. 1067-1082.
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