Potential for utilization of the lipid and DHA-rich fatty acid of integument of squid Ommastrephes bartrami

Lipid and fatty acid compositions of the integument of the squid Ommastrephes bartrami, which is an industrial waste, were assessed for appraisal as a possible new source of phospholipids containing docosahexaenoic acid. Total lipid of wet and dry bases were 2.5 and 14.7%, respectively. Phospholipids was 80, 85% of the total lipid. The major phospholipids were sn-glycero-3-phosphocholine and sn-glycero-3-phosphethanolamine. The sn-glycero-3-phosphocholine consisted of 1,2-diacyl and 1-O-alkyl-2-acyl types, and sn-glycero-3-phosphoethanolamine consisted of 1,2-diacyl, 1-O-alkyl-2-acyl and 1-O-alk-1'-enyl-2-acyl types. Docosahexaenoic acid was a major fatty acid in both 1,2-diacyl and 1-O-alkyl-2-sn-glycero-3-phosphocholine, comprising 49.1 and 77.3 mol% of the total compositional fatty acids, respectively. However, in 2-sn-glycero-3-phosphoethanolamines the major fatty acids were eicosapentaenoic acid (34.8 mol%) in 1,2-diacyl, doxosahexaenoic acid (65.1 mol%) in 1-O-alkyl-2-acyl-, and eicosapentaenoic acid and docosahexaenoic acid (42.9 and 41.2 mol%, respectively) in the 1-O-alkl'-enyl-class. The carbon chain-lengths of the major alkenyl group in 1-O-alk-1'-enyl-2-acyl-sn-glycero-3-phosphoethanolamine were 16 and 18.