Potential of collagen-like triple helical peptides as drug carriers: Their in vivo distribution, metabolism, and excretion profiles in rodents

Hiroyuki Yasui, Chisato M. Yamazaki, Hiroshi Nose, Chihiro Awada, Toshifumi Takao, Takaki Koide

    Research output: Contribution to journalArticle

    17 Citations (Scopus)

    Abstract

    Collagen-model peptides composed of (X-Y-Gly)n sequences were used to study the triple helical structure of collagen. We report the stability of these collagen-like peptides in biological fluids, and their pharmacokinetics including distribution, metabolism, and excretion in animals. A typical collagen-model peptide, H-(Pro-Hyp-Gly)10-OH, was found to be extremely stable in the plasma and distributed mainly in the vascular blood space, and was eliminated through glomerular filtration in the kidneys. Triple helical peptides of (X-Y-Gly)n sequences were quantitatively recovered from the urine of rats after intravenous injection regardless of the differences in peptide net charge between -3 and +6 per triple helix. In contrast, the renal clearance became less efficient when the number of triplet repeats (n) was 12 or more. We also demonstrated the application of a collagen-like triple helical peptide as a novel drug carrier in the blood with a high urinary excretion profile. We further demonstrated that a collagen-like triple helical peptide conjugated to a spin probe, PROXYL, has the potential to evaluate the redox status of oxidative stress-induced animals in vivo.

    Original languageEnglish
    Pages (from-to)705-713
    Number of pages9
    JournalBiopolymers
    Volume100
    Issue number6
    DOIs
    Publication statusPublished - 2013 Nov

    Fingerprint

    Drug Carriers
    Collagen
    Metabolism
    Peptides
    Rodentia
    Animals
    Blood
    Kidney
    Trinucleotide Repeats
    Oxidative stress
    Pharmacokinetics
    Intravenous Injections
    Oxidation-Reduction
    Blood Vessels
    Rats
    Oxidative Stress
    Urine
    Plasmas
    Fluids

    Keywords

    • Collagen
    • Drug delivery
    • Peptide
    • Pharmacokinetics
    • Triple helix

    ASJC Scopus subject areas

    • Biochemistry
    • Biophysics
    • Biomaterials
    • Organic Chemistry

    Cite this

    Potential of collagen-like triple helical peptides as drug carriers : Their in vivo distribution, metabolism, and excretion profiles in rodents. / Yasui, Hiroyuki; Yamazaki, Chisato M.; Nose, Hiroshi; Awada, Chihiro; Takao, Toshifumi; Koide, Takaki.

    In: Biopolymers, Vol. 100, No. 6, 11.2013, p. 705-713.

    Research output: Contribution to journalArticle

    Yasui, Hiroyuki ; Yamazaki, Chisato M. ; Nose, Hiroshi ; Awada, Chihiro ; Takao, Toshifumi ; Koide, Takaki. / Potential of collagen-like triple helical peptides as drug carriers : Their in vivo distribution, metabolism, and excretion profiles in rodents. In: Biopolymers. 2013 ; Vol. 100, No. 6. pp. 705-713.
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