Potential role of lipin-1 in exercise-induced mitochondrial biogenesis

Kazuhiko Higashida, Mitsuru Higuchi, Shin Terada

Research output: Contribution to journalArticle

32 Citations (Scopus)

Abstract

Endurance exercise induces mitochondrial biogenesis in skeletal muscle. It has been shown that lipin-1 acts as a transcriptional coactivator in liver, and stimulates gene expression of mitochondrial enzymes. We hypothesized that lipin-1 might be involved in exercise-induced mitochondrial biogenesis in skeletal muscle. The present investigation first demonstrated that lipin-1 mRNA in rat triceps muscle was increased by approximately 2-fold after an acute bout of endurance swimming exercise. Second, ectopic expression of lipin-1 in L6 myotube increased carnitine palmitoyltransferase-1 and δ-aminolevulinate synthase gene expression. Finally, lipin-1 mRNA expression in rat triceps muscle was significantly elevated at 6 h after subcutaneous injections of 5-aminoimidazole-4-carboxamide ribonucleoside (AICAR) or clenbuterol, which are 5′-AMP-activated protein kinase (AMPK) and β2-adrenergic receptor (β2-AR) activators, respectively. These results may suggest that enhanced expression of lipin-1 is involved in exercise-induced mitochondrial enzyme adaptations, possibly through AMPK- and β2-AR-related mechanisms.

Original languageEnglish
Pages (from-to)587-591
Number of pages5
JournalBiochemical and Biophysical Research Communications
Volume374
Issue number3
DOIs
Publication statusPublished - 2008 Sep 26

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Organelle Biogenesis
Muscle
AMP-Activated Protein Kinases
Gene expression
Adrenergic Receptors
Rats
Skeletal Muscle
Durability
Clenbuterol
Ribonucleosides
Carnitine O-Palmitoyltransferase
Gene Expression
Muscles
Messenger RNA
Skeletal Muscle Fibers
Enzymes
Subcutaneous Injections
Liver
lipine

Keywords

  • β2-adrenergic receptor
  • AICAR
  • AMP-activated protein kinase
  • Clenbuterol
  • Exercise
  • Lipin-1
  • Mitochondria
  • Rat
  • Skeletal muscle

ASJC Scopus subject areas

  • Biochemistry
  • Biophysics
  • Molecular Biology

Cite this

Potential role of lipin-1 in exercise-induced mitochondrial biogenesis. / Higashida, Kazuhiko; Higuchi, Mitsuru; Terada, Shin.

In: Biochemical and Biophysical Research Communications, Vol. 374, No. 3, 26.09.2008, p. 587-591.

Research output: Contribution to journalArticle

Higashida, Kazuhiko ; Higuchi, Mitsuru ; Terada, Shin. / Potential role of lipin-1 in exercise-induced mitochondrial biogenesis. In: Biochemical and Biophysical Research Communications. 2008 ; Vol. 374, No. 3. pp. 587-591.
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