A urea biosensor was developed by immobilizing urease into an electropolymerized electroinactive polypyrrole (PPy) on a platinum electrode. This enzyme-immobilized PPy electrode showed a stable potential response to urea based on the pH response of the electroinactive PPy film electrode. The electropolymerization conditions were optimized, and thus the biosensor showed a Nernstian response with a slope of 31.8mV decade-1 over concentration range of 1 × 10-4-0.3 mol dm-3 urea.
ASJC Scopus subject areas
- Chemical Engineering(all)