Potentiometric detection of serotonin, melatonin, and their precursors/metabolites with monolayer-modified indium tin oxide electrode and their concentration dependency

Zaved Hossain Khan, Takuya Nakanishi, Tetsuya Osaka

Research output: Contribution to journalArticle

8 Citations (Scopus)


A novel potentiometric method using an indium tin oxide (ITO) electrode, surface-treated with aminopropylsilane and disuccinimidyl suberate successively, has been developed for measuring serotonin (5-HT), 5-hydroxy-L-tryptophan (5-HTP), 5-hydroxy-indole-acetic acid (5-HIAA), and melatonin (MT) (N-acetyl-5-methoxytryptamine) in phosphate buffered saline (PBS) solution (pH 7.4). A linear concentration range of 1 nM to 100 μM was obtained with a correlation coefficient of 0.995 for all target analytes. The minimum detectable concentration obtained was 0.1 nM for 5-HT and 1.0 nM for other analytes. The relationship between the molecular structure of the indole derivatives and the enhanced potential shift behaviour was also discussed. This simple and efficient method can be used to measure other neurotransmitters, as well.

Original languageEnglish
Pages (from-to)1849-1852
Number of pages4
JournalSensor Letters
Issue number5
Publication statusPublished - 2011 Oct 1



  • Biosensor
  • Indium tin oxide
  • Melatonin
  • Potentiometric measurement
  • Serotonin

ASJC Scopus subject areas

  • Atomic and Molecular Physics, and Optics
  • Electrical and Electronic Engineering

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