Practical scan speed in atomic force microscopy for live neurons in a physiological solution

Shin Nagayama, Takuro Tojima, Mayumi Morimoto, Shigeo Sasaki, Kazushige Kawabata, Tatsuo Ushiki, Kazuhiro Abe, Etsuro Ito

Research output: Contribution to journalArticle

23 Citations (Scopus)

Abstract

We examined the practical scan speed for the observation of live neurons in a physiological solution using atomic force microscopy (AFM) with a desired vertical resolution of the order of 10-8 m, which was reasonable when taking into account that a flicker of extracellular protein and saccharide on the neurons in the solution occurred during an observation period of a couple of minutes. The practical scan speed was found to be under 40 μm/s, therefore, if we applied AFM using 100 lines and 100 pixels per line to an observation area of 20 μm × 20 μm, the minimum period for acquiring one image was estimated to be about 2 min. This procedure gave us good images that represented the slow three-dimensional dynamics in live neurons, such as the retrograde movement of surface protuberances, but suggested that another approach was required to detect fast structural changes induced by stimulation.

Original languageEnglish
Pages (from-to)3877-3880
Number of pages4
JournalJapanese Journal of Applied Physics, Part 1: Regular Papers and Short Notes and Review Papers
Volume36
Issue number6 SUPPL. B
Publication statusPublished - 1997 Jun
Externally publishedYes

Fingerprint

neurons
Neurons
Atomic force microscopy
atomic force microscopy
protuberances
flicker
carbohydrates
stimulation
Pixels
pixels
proteins
Proteins

Keywords

  • Atomic force microscopy
  • Live neurons
  • Lymnaea
  • Nanoscale resolution
  • Neuron
  • Scan speed
  • Three-dimensional observation
  • Time-dependent change

ASJC Scopus subject areas

  • Physics and Astronomy (miscellaneous)

Cite this

Practical scan speed in atomic force microscopy for live neurons in a physiological solution. / Nagayama, Shin; Tojima, Takuro; Morimoto, Mayumi; Sasaki, Shigeo; Kawabata, Kazushige; Ushiki, Tatsuo; Abe, Kazuhiro; Ito, Etsuro.

In: Japanese Journal of Applied Physics, Part 1: Regular Papers and Short Notes and Review Papers, Vol. 36, No. 6 SUPPL. B, 06.1997, p. 3877-3880.

Research output: Contribution to journalArticle

Nagayama, S, Tojima, T, Morimoto, M, Sasaki, S, Kawabata, K, Ushiki, T, Abe, K & Ito, E 1997, 'Practical scan speed in atomic force microscopy for live neurons in a physiological solution', Japanese Journal of Applied Physics, Part 1: Regular Papers and Short Notes and Review Papers, vol. 36, no. 6 SUPPL. B, pp. 3877-3880.
Nagayama, Shin ; Tojima, Takuro ; Morimoto, Mayumi ; Sasaki, Shigeo ; Kawabata, Kazushige ; Ushiki, Tatsuo ; Abe, Kazuhiro ; Ito, Etsuro. / Practical scan speed in atomic force microscopy for live neurons in a physiological solution. In: Japanese Journal of Applied Physics, Part 1: Regular Papers and Short Notes and Review Papers. 1997 ; Vol. 36, No. 6 SUPPL. B. pp. 3877-3880.
@article{5ac4301069434a609d2f2592e0bb19b2,
title = "Practical scan speed in atomic force microscopy for live neurons in a physiological solution",
abstract = "We examined the practical scan speed for the observation of live neurons in a physiological solution using atomic force microscopy (AFM) with a desired vertical resolution of the order of 10-8 m, which was reasonable when taking into account that a flicker of extracellular protein and saccharide on the neurons in the solution occurred during an observation period of a couple of minutes. The practical scan speed was found to be under 40 μm/s, therefore, if we applied AFM using 100 lines and 100 pixels per line to an observation area of 20 μm × 20 μm, the minimum period for acquiring one image was estimated to be about 2 min. This procedure gave us good images that represented the slow three-dimensional dynamics in live neurons, such as the retrograde movement of surface protuberances, but suggested that another approach was required to detect fast structural changes induced by stimulation.",
keywords = "Atomic force microscopy, Live neurons, Lymnaea, Nanoscale resolution, Neuron, Scan speed, Three-dimensional observation, Time-dependent change",
author = "Shin Nagayama and Takuro Tojima and Mayumi Morimoto and Shigeo Sasaki and Kazushige Kawabata and Tatsuo Ushiki and Kazuhiro Abe and Etsuro Ito",
year = "1997",
month = "6",
language = "English",
volume = "36",
pages = "3877--3880",
journal = "Japanese Journal of Applied Physics, Part 1: Regular Papers & Short Notes",
issn = "0021-4922",
publisher = "Japan Society of Applied Physics",
number = "6 SUPPL. B",

}

TY - JOUR

T1 - Practical scan speed in atomic force microscopy for live neurons in a physiological solution

AU - Nagayama, Shin

AU - Tojima, Takuro

AU - Morimoto, Mayumi

AU - Sasaki, Shigeo

AU - Kawabata, Kazushige

AU - Ushiki, Tatsuo

AU - Abe, Kazuhiro

AU - Ito, Etsuro

PY - 1997/6

Y1 - 1997/6

N2 - We examined the practical scan speed for the observation of live neurons in a physiological solution using atomic force microscopy (AFM) with a desired vertical resolution of the order of 10-8 m, which was reasonable when taking into account that a flicker of extracellular protein and saccharide on the neurons in the solution occurred during an observation period of a couple of minutes. The practical scan speed was found to be under 40 μm/s, therefore, if we applied AFM using 100 lines and 100 pixels per line to an observation area of 20 μm × 20 μm, the minimum period for acquiring one image was estimated to be about 2 min. This procedure gave us good images that represented the slow three-dimensional dynamics in live neurons, such as the retrograde movement of surface protuberances, but suggested that another approach was required to detect fast structural changes induced by stimulation.

AB - We examined the practical scan speed for the observation of live neurons in a physiological solution using atomic force microscopy (AFM) with a desired vertical resolution of the order of 10-8 m, which was reasonable when taking into account that a flicker of extracellular protein and saccharide on the neurons in the solution occurred during an observation period of a couple of minutes. The practical scan speed was found to be under 40 μm/s, therefore, if we applied AFM using 100 lines and 100 pixels per line to an observation area of 20 μm × 20 μm, the minimum period for acquiring one image was estimated to be about 2 min. This procedure gave us good images that represented the slow three-dimensional dynamics in live neurons, such as the retrograde movement of surface protuberances, but suggested that another approach was required to detect fast structural changes induced by stimulation.

KW - Atomic force microscopy

KW - Live neurons

KW - Lymnaea

KW - Nanoscale resolution

KW - Neuron

KW - Scan speed

KW - Three-dimensional observation

KW - Time-dependent change

UR - http://www.scopus.com/inward/record.url?scp=0001657034&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0001657034&partnerID=8YFLogxK

M3 - Article

VL - 36

SP - 3877

EP - 3880

JO - Japanese Journal of Applied Physics, Part 1: Regular Papers & Short Notes

JF - Japanese Journal of Applied Physics, Part 1: Regular Papers & Short Notes

SN - 0021-4922

IS - 6 SUPPL. B

ER -