Practical technique to quantify small, dense low-density lipoprotein cholesterol using dynamic light scattering

Suchin Trirongjitmoah, Kazuya Iinaga, Toshihiro Sakurai, Hitoshi Chiba, Mana Sriyudthsak, Koichi Shimizu

Research output: Contribution to journalArticle

Abstract

Quantification of small, dense low-density lipoprotein (sdLDL) cholesterol is clinically significant. We propose a practical technique to estimate the amount of sdLDL cholesterol using dynamic light scattering (DLS). An analytical solution in a closed form has newly been obtained to estimate the weight fraction of one species of scatterers in the DLS measurement of two species of scatterers. Using this solution, we can quantify the sdLDL cholesterol amount from the amounts of the low-density lipoprotein cholesterol and the high-density lipoprotein (HDL) cholesterol, which are commonly obtained through clinical tests. The accuracy of the proposed technique was confirmed experimentally using latex spheres with known size distributions. The applicability of the proposed technique was examined using samples of human blood serum. The possibility of estimating the sdLDL amount using the HDL data was demonstrated. These results suggest that the quantitative estimation of sdLDL amounts using DLS is feasible for point-of-care testing in clinical practice.

Original languageEnglish
Pages (from-to)265-272
Number of pages8
JournalOptical Review
Volume23
Issue number2
DOIs
Publication statusPublished - 2016 Apr 1

Keywords

  • Dynamic light scattering
  • Fraction
  • Quantification
  • Small, dense low-density lipoprotein

ASJC Scopus subject areas

  • Atomic and Molecular Physics, and Optics

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