Prednisolone causes anxiety- and depression-like behaviors and altered expression of apoptotic genes in mice hippocampus

Yu Kajiyama, Yoshimi Iijima, Shuichi Chiba, Miyako Furuta, Midori Ninomiya, Aiko Izumi, Shigenobu Shibata, Hiroshi Kunugi

Research output: Contribution to journalArticle

18 Citations (Scopus)

Abstract

Glucocorticoids are known to cause psychiatric disorders including depression. Prednisolone (PSL) is one of the most widely used synthetic glucocorticoids to treat various medical diseases; however, little is known about PSL-induced behavioral changes and its molecular basis in the brain. Growing evidence has implicated that hippocampal remodeling or damage play a role in the pathogenic effect of glucocorticoids. In this study, mice were administered PSL (50 or 100 mg/kg) or vehicle for 6 or 7 days and subjected to a series of behavioral tests, i.e., open field, elevated plus maze, prepulse inhibition, forced swim, and tail suspension tests. Hippocampal tissues were subject to microarray analysis using the GeneChip Mouse Genome 430 2.0 Array (Affymetrix) containing 45,101 probes of transcripts. Increased anxiety- and depression-like behaviors assessed with open field, elevated plus maze, and tail suspension tests were observed. Microarray analysis detected 108 transcripts with a fold change of > 2.0 or < 0.5 in which many cell-death-related genes were found. The microarray data was validated by quantitative reverse transcriptase-polymerase chain reaction analysis. Our results demonstrated that PSL causes anxiety- and depression-like behaviors, and suggest that altered gene expressions related to hippocampal remodeling or damage are involved in the effect of PSL on such behaviors.

Original languageEnglish
Pages (from-to)159-165
Number of pages7
JournalProgress in Neuro-Psychopharmacology and Biological Psychiatry
Volume34
Issue number1
DOIs
Publication statusPublished - 2010 Jan 20

Keywords

  • Anxiety
  • Depression
  • Hippocampus
  • Microarray
  • Prednisolone

ASJC Scopus subject areas

  • Pharmacology
  • Biological Psychiatry

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