Preferential immobilization of green fluorescent protein on nano-patterned organosilane templates

Takashi Tanii, Takumi Hosaka, Takeo Miyake, Yuzo Kanari, Guo Jun Zhang, Takashi Funatsu, Iwao Ohdomari

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Abstract

Preferential immobilization of a protein by using a nano-patterned organosilane monolayer as the template was investigated. A passivation film of an organosilane self-assembled monolayer was formed on a silicon dioxide layer by chemical vapor deposition. Then, the inside of the pattern was modified with active chemical groups. At each step, a fluorescent image of the fluophore-labeled biotin, the avidin, and the green fluorescent protein (GFP) was obtained by fluoroscent microscope.

Original languageEnglish
Title of host publicationDigest of Papers - Microprocesses and Nanotechnology 2004
Pages214
Number of pages1
Publication statusPublished - 2004
Event2004 International Microprocesses and Nanotechnology Conference - Osaka
Duration: 2004 Oct 262004 Oct 29

Other

Other2004 International Microprocesses and Nanotechnology Conference
CityOsaka
Period04/10/2604/10/29

Fingerprint

Proteins
Self assembled monolayers
Passivation
Chemical vapor deposition
Monolayers
Microscopes
Silica
Biotin

ASJC Scopus subject areas

  • Engineering(all)

Cite this

Tanii, T., Hosaka, T., Miyake, T., Kanari, Y., Zhang, G. J., Funatsu, T., & Ohdomari, I. (2004). Preferential immobilization of green fluorescent protein on nano-patterned organosilane templates. In Digest of Papers - Microprocesses and Nanotechnology 2004 (pp. 214). [28P-6-63]

Preferential immobilization of green fluorescent protein on nano-patterned organosilane templates. / Tanii, Takashi; Hosaka, Takumi; Miyake, Takeo; Kanari, Yuzo; Zhang, Guo Jun; Funatsu, Takashi; Ohdomari, Iwao.

Digest of Papers - Microprocesses and Nanotechnology 2004. 2004. p. 214 28P-6-63.

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Tanii, T, Hosaka, T, Miyake, T, Kanari, Y, Zhang, GJ, Funatsu, T & Ohdomari, I 2004, Preferential immobilization of green fluorescent protein on nano-patterned organosilane templates. in Digest of Papers - Microprocesses and Nanotechnology 2004., 28P-6-63, pp. 214, 2004 International Microprocesses and Nanotechnology Conference, Osaka, 04/10/26.
Tanii T, Hosaka T, Miyake T, Kanari Y, Zhang GJ, Funatsu T et al. Preferential immobilization of green fluorescent protein on nano-patterned organosilane templates. In Digest of Papers - Microprocesses and Nanotechnology 2004. 2004. p. 214. 28P-6-63
Tanii, Takashi ; Hosaka, Takumi ; Miyake, Takeo ; Kanari, Yuzo ; Zhang, Guo Jun ; Funatsu, Takashi ; Ohdomari, Iwao. / Preferential immobilization of green fluorescent protein on nano-patterned organosilane templates. Digest of Papers - Microprocesses and Nanotechnology 2004. 2004. pp. 214
@inproceedings{fd298c5bbf6d447c9958dfd42bdb6bc5,
title = "Preferential immobilization of green fluorescent protein on nano-patterned organosilane templates",
abstract = "Preferential immobilization of a protein by using a nano-patterned organosilane monolayer as the template was investigated. A passivation film of an organosilane self-assembled monolayer was formed on a silicon dioxide layer by chemical vapor deposition. Then, the inside of the pattern was modified with active chemical groups. At each step, a fluorescent image of the fluophore-labeled biotin, the avidin, and the green fluorescent protein (GFP) was obtained by fluoroscent microscope.",
author = "Takashi Tanii and Takumi Hosaka and Takeo Miyake and Yuzo Kanari and Zhang, {Guo Jun} and Takashi Funatsu and Iwao Ohdomari",
year = "2004",
language = "English",
isbn = "4990247205",
pages = "214",
booktitle = "Digest of Papers - Microprocesses and Nanotechnology 2004",

}

TY - GEN

T1 - Preferential immobilization of green fluorescent protein on nano-patterned organosilane templates

AU - Tanii, Takashi

AU - Hosaka, Takumi

AU - Miyake, Takeo

AU - Kanari, Yuzo

AU - Zhang, Guo Jun

AU - Funatsu, Takashi

AU - Ohdomari, Iwao

PY - 2004

Y1 - 2004

N2 - Preferential immobilization of a protein by using a nano-patterned organosilane monolayer as the template was investigated. A passivation film of an organosilane self-assembled monolayer was formed on a silicon dioxide layer by chemical vapor deposition. Then, the inside of the pattern was modified with active chemical groups. At each step, a fluorescent image of the fluophore-labeled biotin, the avidin, and the green fluorescent protein (GFP) was obtained by fluoroscent microscope.

AB - Preferential immobilization of a protein by using a nano-patterned organosilane monolayer as the template was investigated. A passivation film of an organosilane self-assembled monolayer was formed on a silicon dioxide layer by chemical vapor deposition. Then, the inside of the pattern was modified with active chemical groups. At each step, a fluorescent image of the fluophore-labeled biotin, the avidin, and the green fluorescent protein (GFP) was obtained by fluoroscent microscope.

UR - http://www.scopus.com/inward/record.url?scp=23244445798&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=23244445798&partnerID=8YFLogxK

M3 - Conference contribution

AN - SCOPUS:23244445798

SN - 4990247205

SN - 9784990247201

SP - 214

BT - Digest of Papers - Microprocesses and Nanotechnology 2004

ER -