Preferential reduction of the α-2-6-sialylation from cell surface N-glycans of human diploid fibroblastic cells by in vitro aging

Tomomi Tadokoro, Kiyotaka Yamamoto, Iku Kuwahara, Hirosuke Fujisawa, Masahiko Ikekita, Akiyoshi Taniguchi, Takeshi Sato, Kiyoshi Furukawa

Research output: Contribution to journalArticle

13 Citations (Scopus)

Abstract

Human diploid fibroblastic cell line, TIG-3, has a finite life span of about 80 population doubling levels (PDL), and is used for in vitro aging studies. Young cells (PDL 23) grew to higher cell densities at a higher growth rate than aged cells (PDL 77). When the electrophoretic mobility of cells was determined, the negative surface charge of the aged cells decreased significantly when compared to that of young cells. Lectin blot analysis of membrane glycoproteins showed that the α-2-6-sialylation but not the α-2-3-sialylation of N-glycans decreases markedly in the aged cells when compared to the young cells. In support of this observation, the cDNA microarray assay and reverse transcription-polymerase chain reaction (RT-PCR) analysis showed that the gene expression of the α-2,6-sialyltransferase I (ST6Gal I), which transfers sialic acid to galactose residues of N-glycans, decreases in the aged cells. These results indicate that the concordant decrease of the α-2,6-sialylation of N-glycans with the ST6Gal I gene expression is induced in TIG-3 cells by in vitro aging.

Original languageEnglish
Pages (from-to)443-452
Number of pages10
JournalGlycoconjugate Journal
Volume23
Issue number5-6
DOIs
Publication statusPublished - 2006 Jul 1
Externally publishedYes

Keywords

  • In vitro aging
  • N-glycans
  • ST6Gal I
  • Sialylation
  • TIG-3 cells

ASJC Scopus subject areas

  • Biochemistry
  • Molecular Biology
  • Cell Biology

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