Preliminary Screening of Mutagens with a Microbial Sensor

Isao Karube, Tadashi Matsunaga, Takashi Nakahara, Shuichi Suzuki, Tsuneo Kada

Research output: Contribution to journalArticle

42 Citations (Scopus)

Abstract

Preliminary screening of mutagens Is achieved with a microbial sensor composed of two microbial electrodes using a recombinant deficient strain, Bacillus subtllls (Rec-), and a wild strain, B. subtllls (Rec+). The principle of the sensor Is based on the Inhibitory action of the mutagens on the respiration of B. subtllls (Rec-). When chemical mutagens were added to the system, the current of the Rec- electrode gradually Increased and that of the Rec+ electrode did not Increase. Linear relationships were obtained between the rate of the current increase of the Rec- electrode and the mutagen concentration. Such responses were not obtained when the antibiotics, respiration Inhibitors, and bactericides were employed to the sensor.

Original languageEnglish
Pages (from-to)1024-1026
Number of pages3
JournalAnalytical Chemistry
Volume53
Issue number7
DOIs
Publication statusPublished - 1981
Externally publishedYes

Fingerprint

Mutagens
Screening
Sensors
Microbial electrodes
Electrodes
Bactericides
Bacilli
Anti-Bacterial Agents

ASJC Scopus subject areas

  • Analytical Chemistry

Cite this

Karube, I., Matsunaga, T., Nakahara, T., Suzuki, S., & Kada, T. (1981). Preliminary Screening of Mutagens with a Microbial Sensor. Analytical Chemistry, 53(7), 1024-1026. https://doi.org/10.1021/ac00230a023

Preliminary Screening of Mutagens with a Microbial Sensor. / Karube, Isao; Matsunaga, Tadashi; Nakahara, Takashi; Suzuki, Shuichi; Kada, Tsuneo.

In: Analytical Chemistry, Vol. 53, No. 7, 1981, p. 1024-1026.

Research output: Contribution to journalArticle

Karube, I, Matsunaga, T, Nakahara, T, Suzuki, S & Kada, T 1981, 'Preliminary Screening of Mutagens with a Microbial Sensor', Analytical Chemistry, vol. 53, no. 7, pp. 1024-1026. https://doi.org/10.1021/ac00230a023
Karube I, Matsunaga T, Nakahara T, Suzuki S, Kada T. Preliminary Screening of Mutagens with a Microbial Sensor. Analytical Chemistry. 1981;53(7):1024-1026. https://doi.org/10.1021/ac00230a023
Karube, Isao ; Matsunaga, Tadashi ; Nakahara, Takashi ; Suzuki, Shuichi ; Kada, Tsuneo. / Preliminary Screening of Mutagens with a Microbial Sensor. In: Analytical Chemistry. 1981 ; Vol. 53, No. 7. pp. 1024-1026.
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