Preliminary study on the receptor of gonad-stimulating substance (GSS) as a gonadotropin of starfish

Masatoshi Mita, Kazutoshi Yamamoto, Michiyasu Yoshikuni, Kaoru Ohno, Yoshitaka Nagahama

    Research output: Contribution to journalArticle

    17 Citations (Scopus)

    Abstract

    Previously, the gonad-stimulating substance (GSS) which acts as the gonadotropin was purified from the starfish (Asterina pectinifera) and subsequently, its structure was deciphered. In this study, artificial GSS was synthesized and its interaction with the receptors was examined further. According to competitive experiments using radioiodinated and radioinert GSS in various tissues of A. pectinifera, high specific bindings were observed in the ovarian follicle cells and testicular interstitial cells. Scatchard plot analysis also showed that Kd values were about 4 nM in follicle cells and about 7 nM in interstitial cells. The numbers of binding sites in follicle cells were estimated to be about 3 pmoles/mg protein and in interstitial cells to be about 1 pmoles/mg protein. These strongly suggest that GSS receptors are distributed to follicle cells in female and interstitial cells in male, respectively.

    Original languageEnglish
    Pages (from-to)299-301
    Number of pages3
    JournalGeneral and Comparative Endocrinology
    Volume153
    Issue number1-3
    DOIs
    Publication statusPublished - 2007 Aug

    Fingerprint

    Starfish
    Asteroidea
    gonadotropins
    Gonadotropins
    gonads
    receptors
    Asterina
    Gonads
    Asterina pectinifera
    cells
    Leydig cells
    Ovarian Follicle
    Leydig Cells
    ovarian follicles
    gonad-stimulatingsubstance receptor
    binding sites
    Proteins
    proteins
    Binding Sites

    Keywords

    • Gonad-stimulating substance (GSS)
    • Gonadotropin
    • Oocyte maturation
    • Receptor
    • Starfish

    ASJC Scopus subject areas

    • Endocrinology

    Cite this

    Preliminary study on the receptor of gonad-stimulating substance (GSS) as a gonadotropin of starfish. / Mita, Masatoshi; Yamamoto, Kazutoshi; Yoshikuni, Michiyasu; Ohno, Kaoru; Nagahama, Yoshitaka.

    In: General and Comparative Endocrinology, Vol. 153, No. 1-3, 08.2007, p. 299-301.

    Research output: Contribution to journalArticle

    Mita, Masatoshi ; Yamamoto, Kazutoshi ; Yoshikuni, Michiyasu ; Ohno, Kaoru ; Nagahama, Yoshitaka. / Preliminary study on the receptor of gonad-stimulating substance (GSS) as a gonadotropin of starfish. In: General and Comparative Endocrinology. 2007 ; Vol. 153, No. 1-3. pp. 299-301.
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