Preparation of dehydrated powder of hemoglobin vesicles

Lin Wang, Shinji Takeoka, Eishun Tsuchida*, Satoru Tokuyama, Takahiro Mashiko, Tadashi Satoh

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

10 Citations (Scopus)

Abstract

The carbonyl hemoglobin (CO‐Hb), which was used to prevent denaturation (metHb) during the preparation of samples, was encapsulated into lipid vesicles constituted from unsaturated phospholipid, cholesterol and unsaturated fatty acid. Unsaturated components were polymerized by γ‐irradiation to enhance the stability of bilayer membrane. An aqueous dispersion of resulting Hb vesicles was freeze‐dried in the presence of saccharides (50–200 mM) to obtain a dehydrated powder of Hb vesicles. Change in the vesicle size, the leakage of encapsulated Hb and the oxidation of Hb to metHb were not observed. Therefore, the long‐term storage of Hb vesicles can be realized as a dry powder.

Original languageEnglish
Pages (from-to)17-21
Number of pages5
JournalPolymers for Advanced Technologies
Volume3
Issue number1
DOIs
Publication statusPublished - 1992 Feb

Keywords

  • Freeze‐drying
  • Hemoglobin
  • Polymerized lipids
  • Saccharides
  • Vesicles

ASJC Scopus subject areas

  • Polymers and Plastics

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