Preparation of dehydrated powder of hemoglobin vesicles

Lin Wang, Shinji Takeoka, Eishun Tsuchida, Satoru Tokuyama, Takahiro Mashiko, Tadashi Satoh

    Research output: Contribution to journalArticle

    9 Citations (Scopus)

    Abstract

    The carbonyl hemoglobin (CO‐Hb), which was used to prevent denaturation (metHb) during the preparation of samples, was encapsulated into lipid vesicles constituted from unsaturated phospholipid, cholesterol and unsaturated fatty acid. Unsaturated components were polymerized by γ‐irradiation to enhance the stability of bilayer membrane. An aqueous dispersion of resulting Hb vesicles was freeze‐dried in the presence of saccharides (50–200 mM) to obtain a dehydrated powder of Hb vesicles. Change in the vesicle size, the leakage of encapsulated Hb and the oxidation of Hb to metHb were not observed. Therefore, the long‐term storage of Hb vesicles can be realized as a dry powder.

    Original languageEnglish
    Pages (from-to)17-21
    Number of pages5
    JournalPolymers for Advanced Technologies
    Volume3
    Issue number1
    DOIs
    Publication statusPublished - 1992

    Keywords

    • Freeze‐drying
    • Hemoglobin
    • Polymerized lipids
    • Saccharides
    • Vesicles

    ASJC Scopus subject areas

    • Polymers and Plastics

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