Preparation of human immune effector T cells containing iron-oxide nanoparticles

Hironori Iida, Kosuke Takayanagi, Takuya Nakanishi, Akiko Kume, Kouji Muramatsu, Yoshio Kiyohara, Yasuto Akiyama, Tetsuya Osaka

Research output: Contribution to journalArticle

12 Citations (Scopus)

Abstract

Preparation of human immune T cells containing iron-oxide nanoparticles was carried out for the development of magnetically mediated immunotherapy. Peripheral blood lymphocytes (PBLs) after the incubation with magnetite nanoparticles were found to contain measurable ferric ions, which suggested the incorporation of magnetite nanoparticles. Transmission electron microscopic (TEM) study indicated that the incorporation of magnetite nanoparticles was mediated by endocytosis of PBLs. Furthermore, the effects of dosages and diameter of magnetite nanoparticles on the magnetite incorporation were investigated, and it was demonstrated that the increase in dosage promoted the incorporation of nanoparticles and the uptake into PBLs was more effective for magnetite nanoparticles, which formed smaller aggregations in medium. Finally, the demonstration of magnetite incorporation into enriched T cells and tumor antigen-specific cytotoxic T lymphocyte (CTL) line promises the achievement of magnetically mediated immunotherapy with tumor-specific CTLs containing magnetic nanoparticles.

Original languageEnglish
Pages (from-to)1123-1128
Number of pages6
JournalBiotechnology and bioengineering
Volume101
Issue number6
DOIs
Publication statusPublished - 2008 Dec 15

Keywords

  • Endocytosis
  • Human T cells
  • Immunotherapy
  • Magnetic nanoparticles
  • Transmission electron microscopy (TEM)

ASJC Scopus subject areas

  • Biotechnology
  • Bioengineering
  • Applied Microbiology and Biotechnology

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  • Cite this

    Iida, H., Takayanagi, K., Nakanishi, T., Kume, A., Muramatsu, K., Kiyohara, Y., Akiyama, Y., & Osaka, T. (2008). Preparation of human immune effector T cells containing iron-oxide nanoparticles. Biotechnology and bioengineering, 101(6), 1123-1128. https://doi.org/10.1002/bit.21992