Preparation of human immune effector T cells containing iron-oxide nanoparticles

Hironori Iida, Kosuke Takayanagi, Takuya Nakanishi, Akiko Kume, Kouji Muramatsu, Yoshio Kiyohara, Yasuto Akiyama, Tetsuya Osaka

Research output: Contribution to journalArticle

12 Citations (Scopus)

Abstract

Preparation of human immune T cells containing iron-oxide nanoparticles was carried out for the development of magnetically mediated immunotherapy. Peripheral blood lymphocytes (PBLs) after the incubation with magnetite nanoparticles were found to contain measurable ferric ions, which suggested the incorporation of magnetite nanoparticles. Transmission electron microscopic (TEM) study indicated that the incorporation of magnetite nanoparticles was mediated by endocytosis of PBLs. Furthermore, the effects of dosages and diameter of magnetite nanoparticles on the magnetite incorporation were investigated, and it was demonstrated that the increase in dosage promoted the incorporation of nanoparticles and the uptake into PBLs was more effective for magnetite nanoparticles, which formed smaller aggregations in medium. Finally, the demonstration of magnetite incorporation into enriched T cells and tumor antigen-specific cytotoxic T lymphocyte (CTL) line promises the achievement of magnetically mediated immunotherapy with tumor-specific CTLs containing magnetic nanoparticles.

Original languageEnglish
Pages (from-to)1123-1128
Number of pages6
JournalBiotechnology and Bioengineering
Volume101
Issue number6
DOIs
Publication statusPublished - 2008 Dec 15

Fingerprint

Magnetite Nanoparticles
Magnetite nanoparticles
T-cells
Iron oxides
Nanoparticles
Lymphocytes
T-Lymphocytes
Ferrosoferric Oxide
Blood
Magnetite
Immunotherapy
Tumors
Viral Tumor Antigens
Cytotoxic T-Lymphocytes
Neoplasm Antigens
Antigens
Endocytosis
Demonstrations
Agglomeration
ferric oxide

Keywords

  • Endocytosis
  • Human T cells
  • Immunotherapy
  • Magnetic nanoparticles
  • Transmission electron microscopy (TEM)

ASJC Scopus subject areas

  • Biotechnology
  • Bioengineering
  • Applied Microbiology and Biotechnology

Cite this

Iida, H., Takayanagi, K., Nakanishi, T., Kume, A., Muramatsu, K., Kiyohara, Y., ... Osaka, T. (2008). Preparation of human immune effector T cells containing iron-oxide nanoparticles. Biotechnology and Bioengineering, 101(6), 1123-1128. https://doi.org/10.1002/bit.21992

Preparation of human immune effector T cells containing iron-oxide nanoparticles. / Iida, Hironori; Takayanagi, Kosuke; Nakanishi, Takuya; Kume, Akiko; Muramatsu, Kouji; Kiyohara, Yoshio; Akiyama, Yasuto; Osaka, Tetsuya.

In: Biotechnology and Bioengineering, Vol. 101, No. 6, 15.12.2008, p. 1123-1128.

Research output: Contribution to journalArticle

Iida, H, Takayanagi, K, Nakanishi, T, Kume, A, Muramatsu, K, Kiyohara, Y, Akiyama, Y & Osaka, T 2008, 'Preparation of human immune effector T cells containing iron-oxide nanoparticles', Biotechnology and Bioengineering, vol. 101, no. 6, pp. 1123-1128. https://doi.org/10.1002/bit.21992
Iida H, Takayanagi K, Nakanishi T, Kume A, Muramatsu K, Kiyohara Y et al. Preparation of human immune effector T cells containing iron-oxide nanoparticles. Biotechnology and Bioengineering. 2008 Dec 15;101(6):1123-1128. https://doi.org/10.1002/bit.21992
Iida, Hironori ; Takayanagi, Kosuke ; Nakanishi, Takuya ; Kume, Akiko ; Muramatsu, Kouji ; Kiyohara, Yoshio ; Akiyama, Yasuto ; Osaka, Tetsuya. / Preparation of human immune effector T cells containing iron-oxide nanoparticles. In: Biotechnology and Bioengineering. 2008 ; Vol. 101, No. 6. pp. 1123-1128.
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