Preparation of Liposomes Surface-Loaded with Cationic Manganese Porphyrins as SOD Mimics

Makoto Yuasa, Kenichi Oyaizu, Akihiko Ogata, Noriyoshi Matsukura, Aritomo Yamaguchi

Research output: Contribution to journalArticle

3 Citations (Scopus)

Abstract

A water-soluble cationic 5,10,15,20-tetrakis(N-methylpyridinium-4-yl)porphinatomanganese(III) ion (MnT4MPyP) and water-insoluble 5,10,15,20-tetrakis(N-3-furyl)porphinatomanganese(III) ion (MnT3FuP) were prepared and ion-complexed with an anionic phospholipid membrane to enhance the circulation persistence of the porphyrin complexes in vivo. Fluorescence spectra and fluorescence depolarization experiments indicated MnT3FuP to be situated within the hydrophobic interior of the vesicle composed of the phospholipid membrane, while MnT4MPyP was anchored to the membrane by lipophilic tails with the metalloporphyrin head-group located on the interface between the membrance and aqueous solvent. SOD activity was determined by stopped-flow analysis and cytochrome c assay, which allowed the determination of IC50 and kcat values for the reaction of the metalloporphyrins with superoxide anion radical (O2-·). Sodium stearate-linked MnT4MPyP was the most effective catalyst as an SOD mimic to decompose O2-· at a second-order rate constant of 1.9 × 107 M-1s-1 in dimyristoylphosphatidylcholine (DMPC) liposomes. SOD activity of MnT3FuP was less than that of MnT4MPyP.

Original languageEnglish
Pages (from-to)233-239
Number of pages7
JournalJournal of oleo science
Volume54
Issue number4
DOIs
Publication statusPublished - 2005 Jan 1
Externally publishedYes

Keywords

  • cationic metalloporphyrin
  • liposome
  • nano particle
  • superoxide anion radical
  • superoxide dismutase

ASJC Scopus subject areas

  • Chemistry(all)
  • Chemical Engineering(all)

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