Preparation of luciferase-bacterial magnetic particle complex by artificial integration of MagA-luciferase fusion protein into the bacterial magnetic particle membrane

Tadashi Matsunaga, Atsushi Arakaki, Mikako Takahoko

Research output: Contribution to journalArticle

16 Citations (Scopus)

Abstract

MagA is an iron-translocating protein found in the membranes of magnetic bacterium. Luciferase-bacterial magnetic particle (BMP) complexes were prepared by artificially inserting MagA-luciferase fusion proteins into the membranes of BMPs from Magnetospirillum magneticum strain AMB-1. Fusion proteins were from recombinant Escherichia coli membranes. MagA-Luc fusion proteins were integrated by sonication in vitro. Successful integration of fusion proteins was confirmed by luciferase luminescence on BMPs. Maximum luminescence was obtained after sonication for 3 min with a solution containing 300 mM NaCl, and is 18 times higher compared with recombinant Luc-BMPs generated using previously reported gene fusion techniques.

Original languageEnglish
Pages (from-to)614-618
Number of pages5
JournalBiotechnology and Bioengineering
Volume77
Issue number6
DOIs
Publication statusPublished - 2002 Mar 20
Externally publishedYes

Keywords

  • Anchor protein
  • Bacterial magnetic particles (BMPs)
  • Integration
  • Luciferase
  • MagA

ASJC Scopus subject areas

  • Biotechnology
  • Bioengineering
  • Applied Microbiology and Biotechnology

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