Preparation of luciferase-bacterial magnetic particle complex by artificial integration of MagA-luciferase fusion protein into the bacterial magnetic particle membrane

Tadashi Matsunaga, Atsushi Arakaki, Mikako Takahoko

Research output: Contribution to journalArticle

15 Citations (Scopus)

Abstract

MagA is an iron-translocating protein found in the membranes of magnetic bacterium. Luciferase-bacterial magnetic particle (BMP) complexes were prepared by artificially inserting MagA-luciferase fusion proteins into the membranes of BMPs from Magnetospirillum magneticum strain AMB-1. Fusion proteins were from recombinant Escherichia coli membranes. MagA-Luc fusion proteins were integrated by sonication in vitro. Successful integration of fusion proteins was confirmed by luciferase luminescence on BMPs. Maximum luminescence was obtained after sonication for 3 min with a solution containing 300 mM NaCl, and is 18 times higher compared with recombinant Luc-BMPs generated using previously reported gene fusion techniques.

Original languageEnglish
Pages (from-to)614-618
Number of pages5
JournalBiotechnology and Bioengineering
Volume77
Issue number6
DOIs
Publication statusPublished - 2002 Mar 20
Externally publishedYes

Fingerprint

Bacterial Luciferases
Bacterial Proteins
Luciferases
Sonication
Fusion reactions
Luminescence
Proteins
Membranes
Magnetospirillum
Membrane Fusion Proteins
Recombinant Fusion Proteins
Gene Fusion
Iron
Escherichia coli
Bacteria
Genes

Keywords

  • Anchor protein
  • Bacterial magnetic particles (BMPs)
  • Integration
  • Luciferase
  • MagA

ASJC Scopus subject areas

  • Biotechnology
  • Bioengineering
  • Applied Microbiology and Biotechnology

Cite this

Preparation of luciferase-bacterial magnetic particle complex by artificial integration of MagA-luciferase fusion protein into the bacterial magnetic particle membrane. / Matsunaga, Tadashi; Arakaki, Atsushi; Takahoko, Mikako.

In: Biotechnology and Bioengineering, Vol. 77, No. 6, 20.03.2002, p. 614-618.

Research output: Contribution to journalArticle

@article{67a48c4b788b495190b7f1f12edf2384,
title = "Preparation of luciferase-bacterial magnetic particle complex by artificial integration of MagA-luciferase fusion protein into the bacterial magnetic particle membrane",
abstract = "MagA is an iron-translocating protein found in the membranes of magnetic bacterium. Luciferase-bacterial magnetic particle (BMP) complexes were prepared by artificially inserting MagA-luciferase fusion proteins into the membranes of BMPs from Magnetospirillum magneticum strain AMB-1. Fusion proteins were from recombinant Escherichia coli membranes. MagA-Luc fusion proteins were integrated by sonication in vitro. Successful integration of fusion proteins was confirmed by luciferase luminescence on BMPs. Maximum luminescence was obtained after sonication for 3 min with a solution containing 300 mM NaCl, and is 18 times higher compared with recombinant Luc-BMPs generated using previously reported gene fusion techniques.",
keywords = "Anchor protein, Bacterial magnetic particles (BMPs), Integration, Luciferase, MagA",
author = "Tadashi Matsunaga and Atsushi Arakaki and Mikako Takahoko",
year = "2002",
month = "3",
day = "20",
doi = "10.1002/bit.10114",
language = "English",
volume = "77",
pages = "614--618",
journal = "Biotechnology and Bioengineering",
issn = "0006-3592",
publisher = "Wiley-VCH Verlag",
number = "6",

}

TY - JOUR

T1 - Preparation of luciferase-bacterial magnetic particle complex by artificial integration of MagA-luciferase fusion protein into the bacterial magnetic particle membrane

AU - Matsunaga, Tadashi

AU - Arakaki, Atsushi

AU - Takahoko, Mikako

PY - 2002/3/20

Y1 - 2002/3/20

N2 - MagA is an iron-translocating protein found in the membranes of magnetic bacterium. Luciferase-bacterial magnetic particle (BMP) complexes were prepared by artificially inserting MagA-luciferase fusion proteins into the membranes of BMPs from Magnetospirillum magneticum strain AMB-1. Fusion proteins were from recombinant Escherichia coli membranes. MagA-Luc fusion proteins were integrated by sonication in vitro. Successful integration of fusion proteins was confirmed by luciferase luminescence on BMPs. Maximum luminescence was obtained after sonication for 3 min with a solution containing 300 mM NaCl, and is 18 times higher compared with recombinant Luc-BMPs generated using previously reported gene fusion techniques.

AB - MagA is an iron-translocating protein found in the membranes of magnetic bacterium. Luciferase-bacterial magnetic particle (BMP) complexes were prepared by artificially inserting MagA-luciferase fusion proteins into the membranes of BMPs from Magnetospirillum magneticum strain AMB-1. Fusion proteins were from recombinant Escherichia coli membranes. MagA-Luc fusion proteins were integrated by sonication in vitro. Successful integration of fusion proteins was confirmed by luciferase luminescence on BMPs. Maximum luminescence was obtained after sonication for 3 min with a solution containing 300 mM NaCl, and is 18 times higher compared with recombinant Luc-BMPs generated using previously reported gene fusion techniques.

KW - Anchor protein

KW - Bacterial magnetic particles (BMPs)

KW - Integration

KW - Luciferase

KW - MagA

UR - http://www.scopus.com/inward/record.url?scp=0037139752&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0037139752&partnerID=8YFLogxK

U2 - 10.1002/bit.10114

DO - 10.1002/bit.10114

M3 - Article

C2 - 11807756

AN - SCOPUS:0037139752

VL - 77

SP - 614

EP - 618

JO - Biotechnology and Bioengineering

JF - Biotechnology and Bioengineering

SN - 0006-3592

IS - 6

ER -